Microbial Processes and Products

The development of biotechnology over the last 20 years, and particularly the use of recombinant DNA techniques, has rapidly expanded the opportu- ties for human benefits from living resources. Efforts to reduce pollution, p- vent environmental damage, combat microbial infection, improve food production, and so on can each involve fermentation or the environmental - lease of microorganisms. Many products of fermentation technology, such as alcoholic beverages, bread, antibiotics, amino acids, vitamins, enzymes, and others, have been influenced by the progress of recombinant DNA techniques. The development of new products or the more efficient manufacturing of those already being produced often involve the use of microorganisms as cell fac- ries for many productions and biotransformations. Microbial Processes and Products is intended to provide practical expe- mental laboratory procedures for a wide range of processes and products me- ated by microorganisms. Although not an exhaustive treatise, it provides a detailed “step-by-step” description of the most recent developments in such applied biotechnological processes. The detailed protocols we provide are cross-referenced in the Notes section, contain critical details, lists of problems and their troubleshooting, as well as safety recommendations that may not n- mally appear in journal articles and can be particularly useful for those un- miliar with specific techniques.

Table of Contents:
Development of Improved Strains and Optimization of Fermentation Processes.- Experimental Design in Microbiology.- Metabolic Engineering of Acremonium chrysogenum to Produce Deacetoxycephalosporin C and Bioconversion to 7-Aminodeacetoxycephalosporanic Acid.- Production of Erythromycin With Saccharopolyspora erythraea.- The Phenylacetyl-CoA Catabolon.- Recombinant Microorganisms for the Biosynthesis of Glycosylated Antitumor Compounds.- Assay Methods for Detection and Quantification of Antimicrobial Metabolites Produced by Streptomyces clavuligerus.- Purification of Plasmid DNA Vectors Produced in Escherichia coli for Gene Therapy and DNA Vaccination Applications.- Genome Breeding of an Amino Acid-Producing Corynebacterium glutamicum Mutant.- Metabolic Activity Profiling by 13C Tracer Experiments and Mass Spectrometry in Corynebacterium glutamicum.- Protein and Vitamin Production in Bacillus megaterium.- Strategies for Large-Scale Production of Recombinant Proteins in Filamentous Fungi.- Strain and Culture Conditions Improvement for ?-Carotene Production With Mucor.- Xanthophylls in Fungi.- Methodologies for the Analysis of Fungal Carotenoids.- Insertional Mutagenesis in the Vitamin B2 Producer Fungus Ashbya gossypii.- Improved Polysaccharide Production Using Strain Improvement.- Use of the Morganella morganii phoC Gene as Reporter in Bacterial and Yeast Hosts.- Gene Expression Arrays in Food.- Optimization of Proteome Analysis for Wine Yeast Strains.- Bacteriocin-Producing Strains in a Meat Environment.- In Vitro and In Vivo Interactions of Nonpathogenic Bacteria With Immunocompetent Cells.- Volatile Sulfur Detection in Fermented Foods.- Bioleaching.- Use of PhoN Phosphatase to Remediate Heavy Metals.- Safe Dispatch and Transport of Biological Material.- How to DepositBiological Material for Patent Purposes.- Laws and Regulations for the Protection of Biotechnological Inventions.