Part I: Overview of Microglia Biology

1. A Brief Overview of Multi-Talented Microglia

Bertrand Joseph and Jose Luis Venero

Part II: Isolation and Culture of Microglia

2. Cell Culturing of Human and Murine Microglia Cell Lines

Johanna Rodhe

Jae-Kyung Lee, Malú G Tansey

4. Preparation of Primary Microglia Cultures from Postnatal Mouse and Rat Brains

Tomas Deierborg

5. Isolation of Murine Postnatal Brain Microglia for Phenotypic Characterization Using Magnetic Cell Separation Technology

Ashley S. Harms and Malú G. Tansey

6. Isolation and Culture of Adult Human Microglia within Mixed Glial Cultures for Functional Experimentation and High Content Analysis

Amy M. Smith, Hannah M. Gibbons, Claire Lill, Richard L. M. Faull and Mike Dragunow

Part III: Depletion and Transduction of Microglia

Lorena Pont-Lezica, Sabrina Colasse and Alain Bessis

8. Lentiviral Transduction of Cultured Microglia

Takahiro Masuda, Makoto Tsuda, Hidetoshi Tozaki-Saitoh and Kazuhide Inoue

Part IV: Analysis of Microglial Cytokine Production

9. Microglial Activation: Measurement of Cytokines by Flow Cytometry

Deepak Kumar Kaushik and AnirbanBasu

10. In situ Hybridization of Cytokine mRNA using Alkaline Phosphatase Labelled Oligodeoxynucleotide Probes

Bettina Clausen, Christina Fenger and Bente Finsen

11. Use of Meso-scale Discovery^TM to Examine Cytokinecontentin Microglia Cell Supernatant

Miguel A. Burguillos

Part V: Analysis of Microglia Activation

12. Analysis of Microglial Production of Reactive Oxygen and Nitrogen Species

UrteNeniskyte and Guy C. Brown

13. Quantification of Active Caspase-3 and Active Caspase-8 in Microglia Cells

Edel Kavanagh

14. Quantification of Microglial Phagocytosis by a Flow Cytometer-based Assay

Refik Pul, Chittappen Kandiyil Prajeeth, Martin Stangel

15. Quantification of Microglial Proliferation and Apoptosis by Flow Cytometry

Alicia A. Babcock, Martin Wirenfeldt, and Bente Finsen

16. Fluorescence Imaging of Intracellular Ca²⁺, Na⁺ and H⁺ in Cultured Microglia

Tom Schilling, and Claudia Eder

17. Patch Clamp Protocols to Study Ion Channel Activity in Microglia

Tom Schilling, and Claudia Eder

Sadanand M. Gaikwad and Michael T. Heneka

19. Isolating, Culturing, and Polarizing Primary Human Adult and Fetal Microglia

Bryce A. Durafourt, Craig S. Moore, Manon Blain, and Jack P. Antel

Part VII: Co-culture Systems to Analysis Microglia Interactions with Other Cell Types

20. Understanding Microglia-neuron Cross-talk: Relevance of the Microglia-neuron Co-cultures

Fernando G Correa, Miriam Hernangómez, and Carmen Guaza

21. Preparation of Rodent Primary Cultures for Neuron-Glia, Mixed Glia, Enriched Microglia, and Reconstituted Cultures with Microglia

Shih-Heng Chen, Esteban A. Oyarzabal and Jau-Shyong Hong

Part VIII: Analysis of Microglia Functions in vivo

22. Microglia Detection by Enzymatic Histochemistry

Beatriz Almolda, Berta González and Bernardo Castellano

23. Tomato Lectin Histochemistry for Microglial Visulization

Nàdia Villacampa, Beatriz Almolda, Berta González and Bernardo Castellano

24. Immunohistochemical Detection of Microglia

Rocío M. de Pablos, Ana M. Espinosa-Oliva, and Antonio J. Herrera

25. Intrathecal Infusion of Microglia Cells

Takahiro Masuda, Makoto