1. Microinjection and Micromanipulation: A Historical Perspective
Wenhao Xu
2. Production of Transgenic Mice by Pronuclear Microinjection
Xin-an Pu, Anthony P. Young, and H. Michael Kubisch
3. Transgene Recombineering in Bacterial Artificial Chromosomes
Michael G. Zeidler and Thomas L. Saunders
4. Using TARGATTTM Technology to Generate Site-Specific Transgenic Mice
Ruby Yanru Chen-Tsai
5. Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method
Takuya Sato and Takehiko Ogawa
6. Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer
Yubin Du, Wen Xie, Fan Zhang, and Chengyu Liu
7. Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug Selection
Pinghu Liu, Yan Li, Jingqi Lei, and Lijin Dong
8. Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice
Yubin Du, Wen Xie, Fan Zhang, Uimook Choi, Chengyu Liu, and Colin L. Sweeney
9. Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique
Melissa A. Scott and Yueh-Chiang Hu
10. Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation
Wenning Qin and Haoyi Wang
11. Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides
Ye Liu, Yubin Du, Wen Xie, Fan Zhang, Douglas Forrest, and Chengyu Liu
12. Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection
Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama
13. The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications
Naomi Nakagata, Jorge Sztein, and Toru Takeo
14. The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm
Toru Takeo, Jorge Sztein, and Naomi Nakagata
15. Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line
Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B. Gurumurthy, Masahiro Sato, and Takayuki Shindo
16. Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology
Tuija M. Alcantar and Xin Y. Rairdan
17. Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos
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