1. How to Measure Microtubule Dynamics?

Anne Straube

2. Purification of Tubulin from Porcine Brain

Christopher Gell, Claire T. Friel, Barbara Borgonovo, David N. Drechsel, Anthony A. Hyman, and Jonathon Howard

3. Purification of Tubulin from the Fission Yeast Schizosaccharomyces pombe

Douglas R Drummond, Susan Kain, Anthony Newcombe, Christina Hoey, Miho Katsuki, and Robert A. Cross

4. Generation of Differentially Polyglutamylated Microtubules

Benjamin Lacroix and Carsten Janke

5. Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms

Laurence Lafanechère and Didier Job

6. ab-Tubulin and Microtubule Binding Assays

Jaime N. Campbell and Kevin C. Slep

7. Analysis of Microtubule Assembly Kinetics Using Turbidimetry

Felicia Gaskin

8. Isolation of Centrosomes from Cultured Cells

Simone Reber

9. Preparation of Dual-Color Polarity-Marked Fluorescent Microtubule Seeds

Miho Katsuki, Etsuko Muto, and Rob Cross

10. Reconstitution and Quantification of Dynamic Microtubule End Tracking In Vitro Using TIRF Microscopy

Ivo A. Telley, Peter Bieling, and Thomas Surrey

11. Force Generation by Dynamic Microtubules In Vitro

Svenja-Marei Kalisch, Liedewij Laan, and Marileen Dogterom

12. Variations on the Single Molecule Assay for Microtubule-Associated Proteins and Kinesins

Susanne Bechstedt, Michal Wieczorek, Michael Noujaim, and Gary J. Brouhard

13. Analyzing the ATP Hydrolysis Cycle of Microtubule Motors

Claire T. Friel, Clive R. Bagshaw, and Jonathon Howard

14. Cryo-Electron Tomography of Microtubules Assembled In Vitro from Purified Components

Frédéric M. Coquelle, Sophie Blestel, Claire Heichette, Isabelle Arnal, Charles Kervrann, and Denis Chrétien

15. Kinetochore Microtubules, Analyzed by Correlated Light and Immuno-Electron Microscopy

Andreas Merdes and Jan De Mey

16. Photoactivatable-GFP-a-Tubulin as a Tool to Study Microtubule Plus-End Turnover in Living Human Cells

Catarina P. Samora and Andrew D. McAinsh

17. Quantification of Asymmetric Microtubule Nucleation at Sub-Cellular Structures

Xiaodong Zhu and Irina Kaverina

18. Investigating Microtubule Dynamic Instability Using Microtubule Targeting Agents

Stéphane Honore and Diane Braguer

19. Laser Ablation of the Microtubule Cytoskeleton: Setting Up and Working with an Ablation System

Nicola Maghelli and Iva Tolic-Nørrelykke

20. Isolation, Identification, and Validation of Microtubule-Associated Proteins from Drosophila Embryos

Robin Antrobus and James G. Wakefield

21. Isolation of Novel +TIPs and Their Binding Partners Using Affinity Purification Techniques

Ka Lou Yu, Nanda Keijzer, Casper C. Hoogenraad, and Anna Akhmanova