Part I
1. The Pathway of miRNA Maturation
Michael Sand
2. Micro RNA Maturation and Human Disease
Marlen Hesse and Christoph Arenz
3. Approaches to the Modulation of miRNA Maturation
Valerie T. Tripp, Jaclyn R. McKenna, and Douglas D. Young
Part II
4. Expression Profiling of Components of the miRNA Maturation Machinery
Michael Sand and Marina Skrygan
5. Primary microRNA Processing Assay Reconstituted using Recombinant Drosha and DGCR8
Ian Barr and Feng Guo
6. In-vivo processing Assay Based on a Dual Luciferase Reporter System to Evaluate DROSHA Enzymatic Activity
Vera Bilan, Danilo Allegra, Florian Kuchenbauer, Daniel Mertens
7. Assaying Dicer-mediated miRNA Maturation by Means of Fluorescent Substrates
Marlen Hesse, Brian P. Davies and Christoph Arenz
8. A Fluorescence Correlation Spectroscopy-based Enzyme Assay for Human Dicer
Eileen Magbanua and Ulrich Hahn
9. Detection of Micro RNA Maturation Using Unmodified pre-miRNA and Branched Rolling Circle Amplification
Saskia Neubacher and Christoph Arenz
10. Identification of Inhibitors of microRNA Function from Small Molecule Screens
Colleen M. Connelly and Alexander Deiters
11. Quantitative RT-PCR Specific for Precursor and Mature miRNAs
Hannah Zöllner, Stephan A. Hahn and Abdelouahid Maghnouj.
12. Cellular MicroRNA Sensors Based on Luciferase Reporters
Colleen M. Connelly and Alexander Deiters
13. Inhibition of miRNA maturation by Peptide Nucleic Acids
Concetta Avitabile, Enrica Fabbri, Nicoletta Bianchi, Roberto Gambari and Alessandra Romanelli
14. Molecular Methods for Validation of the Biological Activity of Peptide Nucleic Acids Targeting MicroRNAs
Eleonora Brognara, Enrica Fabbri, Nicoletta Bianchi, Alessia Finotti, Roberto Corradini, and Roberto Gambari
15. Lentiviral Overexpression of miRNAs
Hannah Zöllner, Stephan A. Hahn and Abdelouahid Maghnouj
