"miRNAs in MAPs Computational Analysis" by Swati Srivastava is a comprehensive book that explores the role of miRNAs in gene expression regulation, plant growth, and development, with a particular focus on MAPs (Medicinal and Aromatic Plants). The book describes the computational methods and tools used for analyzing small RNAs, mRNA, and their targets, including target prediction, differential expression analysis, and next-generation sequencing. Data mining and machine learning techniques are also discussed, as well as bioinformatics tools for gene ontology and pathway analysis.
The book covers the impact of miRNAs on plant stress responses, including abiotic and biotic stress, and provides an overview of miRNA biogenesis and the molecular machinery involved, such as Argonaute, Dicer, and Drosha. The use of small RNA sequencing and high-throughput screening methods for the study of miRNAs is also highlighted.
In addition, the book explores the role of miRNAs in plant-microbe interactions and immunity, and discusses the use of system biology and network analysis to understand miRNA-mediated gene regulation. The book also covers RNA interference, post-transcriptional gene silencing, in situ hybridization, qRT-PCR, RNA sequencing, deep sequencing, expression profiling, small RNA libraries, differential expression, evolutionary conservation, functional analysis, and gene function prediction.
Overall, "miRNAs in MAPs Computational Analysis" provides a comprehensive overview of miRNA-mediated gene regulation and the computational methods used to study it, making it an invaluable resource for researchers and students in the field of computational biology and plant science.