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Mitochondrial DNA

Mitochondrial DNA


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About the Book

Part I: Detection of Mitochondrial DNA Mutations and Mitochondrial DNA Damage

1. Comprehensive Mitochondrial Genome Analysis By Massively Parallel Sequencing

Meagan E. Palculict, Victor Wei Zhang, Lee-Jun Wong, and Jing Wang

2. Analysis Of Translesion DNA Synthesis By The Mitochondrial DNA Polymerase g

William C. Copeland, Rajesh Kasiviswanathan, and Matthew J. Longley

3. Quantification Of DNA Damage By Real-Time Qpcr

Wei Wang, Katja Scheffler, Ying Esbensen, And Lars Eide

4. Quantitation Of Mitochondrial DNA Deletions Via Restriction Digestion/Long-Range Single Molecule

PCR

Yevgenya Kraytsberg, Xinhong Guo, Saisai Tao, Alexandra Kuznetsov, Catherine Mclean, Daniel Ehrlich, Evan Feldman, Igor Dombrovsky, Deye Yang, Gregory J. Cloutier, Carmen Castaneda-Sceppa, And Konstantin Khrapko

Part II: Visualization of Mitochondrial DNA and Mitochondrial DNA Nucleoids

5. A Single-Cell Resolution Imaging Protocol Of Mitochondrial DNA Dynamics In Physiopathology, Mtrip, Which Also Evaluates Sublethal Cytotoxicity

Laurent Chatre, Benjamin Montagne and Miria Ricchetti

6. Scalable Isolation Of Mammalian Mitochondria For Nucleic Acid And Nucleoid Analysis

Ken-Wing Lee and Daniel F. Bogenhagen

7. Tracking Mitochondrial DNA In Situ

Anna Ligasová and Karel Koberna

PART II: Analysis of Mitochondrial DNA Replication, Mitochondrial DNA-Encoded Protein Translation and Mitochondrial DNA Copy Number

8. Analysis Of Replicating Mitochondrial DNA By In Organello Labeling And Two-Dimensional Agarose Gel Electrophoresis

Ian J. Holt, Lawrence Kazak, Aurelio Reyes, and Stuart R. Wood

9. Translation And Assembly Of Radiolabelled Mitochondrial DNA-Encoded Protein Subunits From Cultured Cells And Isolated Mitochondria

Luke E. Formosa, Annette Hofer, Christin Tischner, Tina Wenz, and Michael T. Ryan

10. Analysis Of Mitochondrial DNA Copy Number And Its Regulation Through DNA Methylation Of POLGA

Xin Sun, William Lee, Vijesh Vaghjiani, and Justin C. St. John

Part VI: Modification Of Mitochondrial

DNA

11. Engineered Mtzfns For Manipulation Of Human Mitochondrial DNA Heteroplasmy

Payam A. Gammage, Lindsey Van Haute, and Michal Minczuk

12. Generation Of Xenomitochondrial Embryonic Stem Cells For The Production Of Live Xenomitochondrial Mice

Ian A. Trounce, Jessica Ackerley, and Matthew McKenzie

13. Import Of Fluorescent RNA Into Mitochondria Of Living Cells

Jaroslav Zelenka and Petr Jezek

Part V: Purification of Proteins Involved In Mitochondrial DNA Replication and Transcription

14. Purification And Comparative Assay Of The Human Mitochondrial Replicative DNA Helicase

Fernando A. Rosado-Ruiz, Minyoung So, and Laurie S. Kaguni

15. Expression And Purification Of Mitochondrial RNA Polymerase And Transcription Factor A From Drosophila Melanogaster

John P. Gajewski, Jamie J. Arnold, Tiina S. Salminen, Laurie S. Kaguni, and Craig E. Cameron

16. Purification And Comparative Assay Of Human Mitochondrial Single-Stranded DNA-Binding Protein

Grzegorz L. Ciesielski, Fernando A. Rosado-Ruiz, and Laurie S. Kaguni

17. Biolayer Interferometry: A Novel Method To Elucidate Protein- Protein And Protein- DNA Interactions In The Mitochondrial DNA Replisome

Grzegorz L. Ciesielski, Vesa P. Hytönen, and Laurie S. Kaguni

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Product Details
  • ISBN-13: 9781493949441
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Edition: Softcover reprint of the original 3rd ed. 2016
  • Language: English
  • Returnable: Y
  • Spine Width: 13 mm
  • Weight: 494 gr
  • ISBN-10: 1493949446
  • Publisher Date: 23 Aug 2016
  • Binding: Paperback
  • Height: 254 mm
  • No of Pages: 234
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm
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