1. Mitochondrial Dysfunction in Mitochondrial Medicine: Current Limitations, Pitfalls, and Tomorrow
Naig Gueguen, Guy Lenaers, Pascal Reynier, Volkmar Weissig, and Marvin Edeas
2. Preparation of 'Functional' Mitochondria - A Challenging Business
Stefan Lehr, Sonja Hartwig, and Jorg Kotzka
3. Isolation and Quality Control of Functional Mitochondria
Sonja Hartwig, Jorg Kotzka, and Stefan Lehr
4. Purification of Functional Platelet Mitochondria Using a Discontinuous Percoll Gradient
Jacob L. Léger, Nicolas Pichaud, and Luc H. Boudreau
5. Mechanical Permeabilization as a New Method for Assessment of Mitochondrial Function in Insect Tissues
Alessandro Gaviraghi, Yan Aveiro, Stephanie S. Carvalho, Rodiesley S. Rosa, Matheus P. Oliveira, and Marcus F. Oliveira
6. Analysis of Mitochondrial Retrograde Signaling In Yeast Model Systems
Nicoletta Guaragnella, Masa Zdralevic, Zdena Palková, and Sergio Giannattasio
7. Native Gel Electrophoresis and Immunoblotting to Analyze Electron Transport Chain Complexes Gisela Beutner and George A. Porter, Jr.
8. Measuring Mitochondrial Hydrogen Peroxide Levels and Glutathione Redox Equilibrium in Drosophila Neuron Subtypes Using Redox-Sensitive Fluorophores and 3D Imaging
Lori M. Buhlman, Petros P. Keoseyan, Kathryn Houlihan, and Amber N. Juba
9. Assessment of Mitochondrial Cell Metabolism By Respiratory Chain Electron Flow Assays
Flavia Radogna, Déborah Gérard, Mario Dicato, and Marc Diederich
10. Whole-Cell and Mitochondrial dNTP Pool Quantification from Cells and Tissues Juan C. Landoni, Liya Wang, and Anu Suomalainen
11. Single Particle-Tracking Method in Fluorescence Microscopy to Monitor Bioenergetic Responses Of Individual Mitochondria
Camille Colin, Emmanuel Suraniti, Emma Abell, Audrey Sémont, Neso Sojic, Philippe Diolez, and Stéphane Arbault
12. Investigation of Mitochondrial ADP-ribosylation via Immunofluorescence
Ann-Katrin Hopp and Michael O. Hottiger
13. Assessment of Mitochondrial Ca2+ Uptake
András T. Deak, Claire Jean-Quartier, Alexander I. Bondarenko, Lukas N. Groschner, Roland Malli, Wolfgang F. Graier, and Markus Waldeck-Weiermair
14. Assessment of Mitochondrial Membrane Potential and NADH Redox State in Acute Brain Slices
Andrey Y. Vinokurov, Viktor V. Dremin, Gennadii A. Piavchenko, Olga A. Stelmashchuk, Plamena R. Angelova, and Andrey Y. Abramov
15. Evaluation of Mitochondria Content and Function in Live Cells by Multi-color Flow Cytometric Analysis
Hsiu-Han Fan, Tsung-Lin Tsai, Ivan L. Dzhagalov, and Chia-Lin Hsu
16. Analysis of Mitochondrial Dysfunction during Cell Death
Vladimir Gogvadze and Boris Zhivotovsky
17. Modified Blue-Native Gel Approach for Analysis of Respiratory Super-Complexes
Sergiy M. Nadtochiy, Megan Ngai, and Paul S. Brookes
18. Patch-Clamp Recording of the Activity of Ion Channels in the Inner Mitochondrial Membrane
Piotr Bednarczyk, Rafal P. Kampa, Shur Kucman, Aleksandra Sęk, Agnieszka Walewska, and Piotr Koprowski
19. Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway
Ana S. Almeida, Cláudia Figueiredo-Pereira, and Helena L.A. Vieira
20. 3D Optical Cryo-Imaging Method: A Novel Approach To Quantify Renal Mitochondrial Bioenergetics Dysfunction
Shima Mehrvar, Amadou K. S. Cama
