The Mitotic Exit Network by Fernando Monje-Casas
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The Mitotic Exit Network

The Mitotic Exit Network


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About the Book

Part I: Analysis of Cell Cycle Progression and Mitotic Exit

1. Regulation of Mitotic Exit in Saccharomyces cerevisiae

Bàrbara Baro, Ethel Queralt, and Fernando Monje-Casas

2. Methods of Synchronization of Yeast Cells for the Analysis of Cell Cycle Progression

M. Angeles Juanes

3. Analysis of Cell Cycle Progression in Saccharomyces cerevisiae Using the Budding Index and Tubulin Staining

Marta Muñoz-Barrera and Fernando Monje-Casas

4. Determination of Cell Cycle Stage and Mitotic Exit Through the Quantification of the Protein Levels of Known Mitotic Regulators

Cristina Cepeda-García

5. Cdc14 Localization as a Marker for Mitotic Exit: In Vivo Quantitative Analysis of Cdc14 Release

Gabriel Neurohr and Manuel Mendoza

Part II: Biochemistry of the MEN

6. In Vitro Analysis of Tem1 GTPase Activity and Regulation by the Bfa1/Bub2 GAP

Marco Geymonat, Adonis Spanos, and Katrin Rittinger

7. Analysis of Protein-Protein Interactions Between MEN Components

Rami Rahal

8. A New Methodology for the Quantification of In Vivo Cdc14 Phosphatase Activity

Ethel Queralt and José-Antonio Rodriguez-Rodriguez

9. Analysis of SUMOylation in the RENT Complex by Fusion to a SUMO-Specific Protease Domain

Neus Colomina, Clàudia Guasch, and Jordi Torres-Rosell

10. A Substrate Trapping Method for Identification of Direct Cdc14 Phosphatase Targets

Brendan L. Powers, Hana Hall, Harry Charbonneau, and Mark C. Hall

Part III: Analysis of the Localization of MEN Components

11. Localizing MEN Components by Indirect Immunofluorescence Analysis of Budding Yeast

Francesca Senic-Matuglia and Rosella Visintin

12. Analysis of the Localization of MEN Components by Live Cell Imaging Microscopy

Zhiang Guo and Marisa Segal

13. Evaluation of the Dynamicity of Mitotic Exit Network and Spindle Position Checkpoint Components on Spindle Pole Bodies by Fluorescence Recovery After Photobleaching (FRAP)

Ayse Koca Caydasi and Gislene Pereira

14. Asymmetric Localization of Components and Regulators of the Mitotic Exit Network at Spindle Pole Bodies

Ilaria Scarfone and Simonetta Piatti

15. Evaluation of the Nucleolar Localization of the RENT Complex to Ribosomal DNA by Chromatin Immunoprecipitation Assays

Julie Huang, Nahid Iglesias, and Danesh Moazed

Part IV: Control of Cellular Processes by the MEN

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Product Details
  • ISBN-13: 9781493982202
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Height: 254 mm
  • No of Pages: 281
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm
  • ISBN-10: 1493982206
  • Publisher Date: 27 Jun 2018
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Spine Width: 15 mm
  • Weight: 557 gr

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