Molecular Characterization of Orientia From Patients by Manigandan C Manigandan
Home > Parenting, Family & Health > Family & health > Molecular Characterization of Orientia From Patients
Molecular Characterization of Orientia From Patients

Molecular Characterization of Orientia From Patients


     0     
5
4
3
2
1



Out of Stock


Notify me when this book is in stock
About the Book

Scrub typhus is a zoonotic infection spread by vectors and can manifest as an acute febrile illness with a potentially severe outcome. The causative agent, Orientia tsutsugamushi, is an obligate intracytoplasmic bacterium that is transmitted by the larval stage of trombiculid mites. There are nymphal and adult trombiculid mites in the soil. There is only one life stage that can transmit the disease to humans. After a chigger bites you, you may develop an eschar, a type of lesion that may cause fever, headache, myalgia, nausea, vomiting, diarrhoea, cough, or breathlessness. Tsutsugamushi O., unlike many other bacterial pathogens, can only survive within a host cell, and thus it can only infect phagocytic cells. The organism also infects non-phagocytic cells, such as endothelial cells, fibroblasts, and phagocytic cells, such as macrophages and polymorphs. Scrub typhus is an important rickettsial infection, but is often under-recognized. Weitzel et al. estimate that more than a million cases are affected annually. Furthermore, over a billion cases are at risk for this febrile illness worldwide. Despite the availability of serological tests, they fail to diagnose typhus at an early stage due to insufficient production of antibodies and need for frequent follow-up tests. It can take at least a month for the results of the isolation of O. tsutsugamushi.


Thus, isolation in culture is not appropriate for the routine diagnosis of scrub typhus (Koh et al., 2010). There is still a possibility of missing out on the diagnosis, which is why there is a need for an alternative diagnostic method. PCR makes the DNA of the agent millions of times stronger. This means that O. tsutsugamushi DNA can be detected in blood and tissue samples with great sensitivity using PCR. The sensitivity of this assay has been enhanced by the development of the nested PCR (nPCR) assays, which are performed using two separate amplification steps. Several recent studies from India (Kumar et al., 2014 and Sinha et al., 2014) and elsewhere (Ruang-areerate et al., 2011 and Izzard et al., 2010) have shown that nPCR targeting the 56kDa type-specific protein gene is effective. The 56kDa gene is a type-specific protein antigen gene, which is specific to O. tsutsugamushi.


This protein has four hypervariable domains that are responsible for the antigenic variation (Blacksell et al., 2008). The 56kDa type-specific antigen (TSA) is a major outer membrane protein of the organism and is involved in penetration into the host cells. It induces strong humoral immunity. It contains both group-specific and type-specific epitopes, which may be useful for the diagnosis of scrub typhus. To diagnose typhus from the study population, we planned to target the 56kDaProtein antigen gene.



Best Sellers



Product Details
  • ISBN-13: 9789927068911
  • Publisher: Aruna Publishing House
  • Binding: Paperback
  • Language: English
  • Returnable: Y
  • Weight: 281 gr
  • ISBN-10: 9927068913
  • Publisher Date: 18 Dec 2022
  • Height: 229 mm
  • No of Pages: 166
  • Spine Width: 9 mm
  • Width: 152 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Molecular Characterization of Orientia From Patients
Aruna Publishing House -
Molecular Characterization of Orientia From Patients
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Molecular Characterization of Orientia From Patients

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals



    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!