About the Book
1. Applications of Molecular Genetics to the Study of Asthma
Catalina S. Sanz Lozano, Virginia García-Solaesa, Ignacio Dávila González, and María Isidoro-García
2. Assessment and Validation of New Genetic Variants: A systematic In Silico Approach
Belén García Berrocal, Asunción García-Sánchez, and María Isidoro-García
3. Introduction to the Gene Expression Analysis Ignacio San Segundo-Val and Catalina S. Sanz Lozano
4. Real Time PCR for Gene Expression Quantification in Asthma
Ignacio San Segundo-Val, Virginia García-Solaesa, and Asunción García-Sánchez
5. Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma
Ignacio San Segundo-Val and Catalina S. Sanz Lozano
6. Review of Methods to Study Gene Expression Regulation Applied to Asthma
Asunción García-Sánchez and Fernando Marqués García
7. Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma
Virginia García-Solaesa and Catalina S. Sanz Lozano
8. SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma
Virginia García-Solaesa and Sara Ciria Abad
9. Chromatin Immunoprecipitation: Application to the Study of Asthma
Asunción García-Sánchez and Fernándo Marqués-García
10. Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols
Asunción García-Sánchez and Fernando Marqués-García
11. Protocols for Exosome Isolation and RNA Profiling
Fernando Marqués-García and María Isidoro-García
12. Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell
Elena Marcos-Vadillo and Asunción García-Sánchez
13. Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line
Elena Marcos-Vadillo and Asunción García-Sánchez
14. Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line
Elena Marcos-Vadillo and Asunción García-Sánchez
15. Review of Mouse Models Applied to the Study of Asthma
Fernando Marqués-García and Elena Marcos-Vadillo
16. General and Specific Mouse Models for Asthma Research
Fernando Marqués-García and Elena Marcos-Vadillo
17. Mouse Models applied to the Research of Pharmacological Treatments in Asthma
Fernando Marqués-García and Elena Ma