Monoclonal Antibodies
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Monoclonal Antibodies: Hybridomas: A New Dimension in Biological Analyses

Monoclonal Antibodies: Hybridomas: A New Dimension in Biological Analyses

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International Edition


About the Book

On August 7, 1975, Kohler and Milstein published in Nature (256:495) a report describing "Continuous cultures of fused cells secreting antibody of predefined specificity. " Their report has become a classic and has already had a profound effect on basic and applied research in biology and medicine. By the time the first Workshop on Lymphocyte Hybridomas (Current Topics in Microbiology and Im­ munology 81, 1978) was held on April 3-5, 1978, in Bethesda, Maryland, investi­ gators from many laboratories had made hybrids between plasmacytomas and spleen cells from immunized animals and had obtained monoclonal antibodies reacting with a broad variety of antigenic determinants. At the time Kohler and Milstein introduced this new technology, the editors of this volume were involved in the production of antisera against differentiation antigens (K. B. B. ), histocompatibility antigens (T. ]. McK. ), and human tumor­ associated antigens (R. H. K. ). Because of the potential usefulness of monoclonal antibodies in these areas, we each began production of hybridomas and analysis of the resulting monoclonal reagents. One of the most interesting aspects of participation in the early stages of the development and application of hybrid­ oma technology has been observing how the implications of the initial observa­ tions gradually spread first among the practitioners of immunology and immu­ nogenetics, and then to other areas of the biological sciences, such as developmental biology, biochemistry, human genetics, and cell and tumor biology.

Table of Contents:
I Introduction Production of Specific Antibodies from Continuous Cell Lines in Vitro.- 1 Plasmacytomas and Hybridomas Development and Applications.- 2 Continuously Proliferating Human Cell Lines Synthesizing Antibody of Predetermined Specificity.- II Analysis of Immunoglobulin Structure and Genetics.- 3 Antibody Diversity Patterns and Structure of Idiotypic Determinants on Murine Anti- ?(1 ? 3) Dextran Antibodies.- 4 Defining the B-Cell Repertoire with Hybridomas Derived from Monoclonal Fragment Cultures.- 5 The Use of Hybridomas to Localize Mouse Immunoglobulin Genes.- III Detection and Analysis of Human Gene Products.- 6 Monoclonal Antibodies as Tools for Human Genetic Analysis.- 7 The Use of Hybridomas in Enzyme Genetics.- 8 Characterization of a Human T-Cell Population Identified and Isolated by a Monoclonal Antibody.- 9 Mouse × Human Hybridomas.- 10 Monoclonal Antibodies against Human Tumor-Associated Antigens.- IV Monoclonal Antibodies as Probes in the Study of Cellular Differentiation and Immunogenetics.- 11 Germ-Cell-Related and Nervous-System-Related Differentiation and Tumor Antigens.- 12 Cell-Surface Differentiation in the Mouse Characterization of “Jumping” and “Lineage” Antigens Using Xenogeneic Rat Monoclonal Antibodies.- 13 Rat-Mouse Hybridomas and Their Application to Studies of the Major Histocompatibility Complex.- 14 Murine T-Cell Differentiation Antigens Detected by Monoclonal Antibodies.- 15 Monoclonal Antibodies That Define T-Lymphocyte Subsets in the Rat.- 16 Monoclonal Antibody Therapy of Mouse Leukemia.- V Monoclonal Antibodies to Microorganisms.- 17 Mapping of Viral Proteins with Monoclonal Antibodies Analysis of the Envelope Proteins of Murine Leukemia Viruses.- 18 Monoclonal Antibodies against Influenza Virus.- 19 MonoclonalAntibodies against Rabies Virus.- 20 Monoclonal Antibodies against Streptococcal Antigens.- Appendix Methods for Production and Characterization of Monoclonal Antibodies.- Plasmacytoma Cell Lines.- Fusion by Centrifugation of Cells Suspended in Polyethylene Glycol.- Roger H. Kennett.- Fusion of Cells in an Adherent Monolayer.- Thomas J. McKearn.- Fusion of Cells in Suspension and Outgrowth of Hybrids in Conditioned Medium.- Walter Gerhard.- Cloning of Hybridomas Cloning in Semisolid Agarose.- Roger H. Kennett.- Cloning of Hybridoma Cells by Limiting Dilution in Fluid Phase.- Thomas J. McKearn.- Freezing of Hybridoma Cells.- Roger H. Kennett.- Enzyme-Linked Antibody Assay with Cells Attached to Polyvinyl Chloride Plates.- Roger H. Kennett.- Peroxidase-Conjugated Antiglobulin Method for Visual Detection of Cell-Surface Antigens.- Zdenka L. Jonak.- Radioimmunoassay.- Kathleen B. Bechtol.- Quantitative Absorption/Blocking Assay.- Kathleen B. Bechtol.- Radioimmunoassay for Genetic Screening of Proteins Using Monoclonal Antibodies.- C. A. Slaughter, M. C. Coseo, C. Abrams, M. P. Cancro, and H. Harris.- Binding of Hybridoma Antibodies to Polyvinyl Chloride Microtiter Dishes.- Thomas J. McKearn.- Microcytotoxicity Assay.- Roger H. Kennett.- Thomas J. McKearn.- Immunoprecipitation with Monoclonal Antibodies.- Lois A. Lampson.- Binding Inhibition Using Biosynthetically Labeled Monoclonal Antibody.- Lois A. Lampson.- Protein lodination Suitable for Labeling Hybridoma Antibodies.- Dennis M. Klinman and J. C. Howard.- Method for Growing Hybridomas in Rats or Mice.- Thomas J. McKearn.- Zdenka L. Jonak.- Characterization of Hybridoma Immunoglobulins by Sodium Dodecylsulf ate-Polyacrylamide Gel Electrophoresis.- J. B. Haas and Roger H. Kennett.- The Calculation of Antigen-Antibody Binding Constants by Radioimmunoassay.- Mark E. Frankel.- References to Other Methods.


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Product Details
  • ISBN-13: 9781461575078
  • Publisher: Springer-Verlag New York Inc.
  • Publisher Imprint: Springer-Verlag New York Inc.
  • Height: 254 mm
  • No of Pages: 424
  • Returnable: Y
  • Width: 178 mm
  • ISBN-10: 1461575079
  • Publisher Date: 30 Mar 2012
  • Binding: Paperback
  • Language: English
  • Returnable: Y
  • Sub Title: Hybridomas: A New Dimension in Biological Analyses


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