Monoclonal Antibodies

Part I: Antibody Generation

1. Antigen Production for Monoclonal Antibody Generation

Giovanni Magistrelli, Pauline Malinge

2. Method for the Generation of Antibodies Specific for Site- and Post-translational Modifications

3. Immunization, Hybridoma Generation and Selection for Monoclonal Antibody Production

Hyung-Yong Kim, Alexander Stojadinovic, and Mina J. Izadjoo

4. Hybridoma Technology for the Generation of Rodent mAbs via Classical Fusion

Efthalia Chronopoulou, Alejandro Uribe-Benninghoff, Cindi R. Corbett, and Jody D. Berry

5. Generation of Rabbit Monoclonal Antibodies

Pi-ChenYam and Katherine L. Knight

6. Screening Hybridomas for Cell Surface Antigens by High-throughput Homogenous Assay and Flow Cytometry

Alejandro Uribe-Benninghoff, Teresa Cabral, Efthalia Chronopoulou, Jody D. Berry and Cindi R. Corbett

7. Screening and Subcloning of High Producer Transfectomas using Semi-solid Media and Automated Colony Picker

Suba Dharshanan and Cheah Swee Hung

8. Design and Generation of Synthetic Antibody Libraries for Phage Display

Gang Chen and Sachdev S. Sidhu

9. Selection and Screening using Antibody Phage Display Libraries

Patrick Koenig and Germaine Fuh

10. Yeast Surface Display for Antibody Isolation: Library Construction, Library Screening, and Affinity Maturation

James A. Van Deventer and Karl Dane Wittrup

11. Human B Cell Immortalization for Monoclonal Antibody Production

Joyce Hui-Yuen, Siva Koganti, and Sumita Bhaduri-McIntosh

12. Using Next-generation Sequencing for Discovery of High-frequency Monoclonal Antibodies in the Variable Gene Repertoires from Immunized Mice

Ulrike Haessler and Sai T. Reddy

Part II: Antibody Expression and Purification

13. Cloning, Reformatting, and Small Scale Expression of Monoclonal Antibody Isolated from Mouse, Rat, or Hamster Hybridoma

Jeremy Loyau and François Rousseau

14. Cloning of Recombinant Monoclonal Antibodies from Hybridomas in a Single Mammalian Expression Plasmid

Nicole Müller-Sienerth, Cécile Crosnier, Gavin J. Wright and Nicole Staudt

15. Monoclonal Antibody Purification by Ceramic Hydroxyapatite Chromatography

Larry J. Cummings, Russell G. Frost and Mark A. Snyder

16. Rapid Purification of Monoclonal Antibodies using Magnetic Microspheres

Pauline Malinge, Giovanni Magistrelli

17. Generation of Cell Lines for Monoclonal Antibody Production

Krista Alvin and Jianxin Ye

18. Expression and Purification of Recombinant Antibody Formats and Antibody Fusion Proteins

Martin Siegemund, Fabian Richter, Oliver Seifert, Felix Unverdorben and Roland E. Kontermann

19. Purification of Antibodies and Antibody Fragments using CaptureSelect Affinity Resins

Pim Hermans, Hendrik Adams, Frank Detmers

20. Reformatting of scFv antibodies into the scFv-Fc format and their Downstream Purification

Emil Bujak, Mattia Matasci, Dario Neri, and Sarah Wulhfard

Part III: Anitbody Characterization and Modification

21. Antibody V and C Domain Sequence, Structure, and Interaction Analysis with Special Reference to IMGT(R)

Eltaf Alamyar, Véronique Giudicelli, Patrice Duroux and Marie-Paule Lefranc

22. Measuring Antibody Affinities as well as the Active Concentration of Antigens Present on a Cell Surface

Palaniswami Rathanaswami

23. Determination of Antibody Structures

Robyn L. Stanfield

24. Affinity Maturation of Monoclonal Antibodies by Multi Site-Directed Mutagenesis

Hyung-Yong Kim, Alexander Stojadinovic, and Mina J. Izadjoo

25. Epitope Mapping with Membrane-bound Synthetic Overlapping Peptides

Terumi Midoro-Horiuti and Randall M. Goldblum

26. Epitope Mapping by Epitope Excision, Hydrogen/Deuterium Exchang