1. Transgenic Mouse Models
Jennifer Skelton, Linda H. Gower, Anne McGough, and Patricia A. Labosky
2. Pulmonary Antigen Presenting Cells: Isolation, Purification, and Culture
Hideki Nakano and Donald N. Cook
3. Evaluation of T Cell Function in Allergic Disease
Brianne R. Barker
4. Evaluating B-Cells: From Bone Marrow Precursors to Antibody Producing Cells
M. Manuela Rosado, Marco Scarsella, Simona Cascioli, Ezio Giorda, and Rita Carsetti
5. Protocols for Identifying, Enumerating and Assessing Mouse Eosinophils
Kimberly D. Dyer, Katia E. Garcia-Crespo, Caroline M. Percopo, Eva M. Sturm, and Helene F. Rosenberg
6. Evaluation of Classical, Alternative, and Regulatory Functions of Bone Marrow-Derived Macrophages
Beckley K. Davis
7. Applications of Mouse Airway Epithelial Cell Culture for Asthma Research
Amjad Horani, John D. Dickinson, and Steven L. Brody
8. Isolation and Characterization of Mast Cells in Mouse Models of Allergic Diseases
Martina Kovarova
9. Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE
Jamie L. Sturgill and Daniel H. Conrad
10. Protocols for the Induction and Evaluation of Systemic Anaphylaxis in Mice
Elizabeth Doyle, Julia Trosien, and Martin Metz
11. Contact Hypersensitivity Models in Mice
Irving C. Allen
12. Induction of Allergic Rhinitis in Mice
Virginia McMillan Carr and Alan M. Robinson
13. Induction of Allergic Airway Disease Using House Dust Mite Allergen
Irving C. Allen
14. An Inhalation Model of Allergic Fungal Asthma: Aspergillus fumigatus-Induced Inflammation and Remodeling in Allergic Airways Disease
Jane M. Schuh and Scott A. Hoselton
15. PAMPs and DAMPs in Allergy Exacerbation Models
Monique A.M. Willart, Philippe Poulliot, Bart N. Lambrecht, and Mirjam Kool
16. Assessment of Airway Hyperresponsiveness in Mouse Models of Allergic Lung Disease Using Detailed Measurements of Respiratory Mechanics
John M. Hartneyand Annette Robichaud
17. Bilateral Vagotomy as a Tool for Determining Autonomic Involvement in Airway Responses in Mouse Models of Asthma
Jaime M. Cyphert
18. Clara Epithelial Cell Depletion in the Lung
Sanchaita S. Sonar and Jan C. Dudda
19. A Mouse Model for Evaluating the Contribution of Fibrocytes and Myofibroblasts to Airway Remodeling in Allergic Asthma
Matthias Schmidt and Sabrina Mattoli
20. Assessment of Airway Hyperresponsiveness in Murine Tracheal Rings
Jeremiah T. Herlihy, Iurii Semenov, and Robert Brenner
21. Use of the Cockroach Antigen Model of Acute Asthma to Determine the Immunomodulatory Role of Early Exposure to Gastrointestinal Infection
Carolyn G. Durham, Lisa M. Schwiebert, and Robin G. Lorenz
22. Expression Profiling to Identify Candidate Genes Associated with Allergic Phenotypes
Willie June Brickey
23. Flow Cytometric Methods for the Assessment of Allergic Disease
Adeeb H. Rahman
24. Generation of Bone Marrow and Fetal Liver Chimeric Mice
Eda K. Holl