1. In situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional â-galactosidase Staining
Yoshihiro Komatsu, Satoshi Kishigami, and Yuji Mishina
2. Two Color In Situ Hybridization of Whole-Mount Mouse Embryos
Kristin K. Biris and Terry P. Yamaguchi
3. Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosections
Davide De Pietri Tonelli, Yoanne M. Clovis and Wieland B. Huttner
4. Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray Assays
Latasha C. Redmond, Christopher J. Pang, Catherine Dumur, Jack L. Haar and Joyce A. Lloyd
5. EMAGE: Electronic Mouse Atlas of Gene Expression
Lorna Richardson, Peter Stevenson, Shanmugasundaram Venkataraman. Yiya Yang, Nick Burton, Jianguo Rao, Jeffrey H. Christiansen, Richard A. Baldock and Duncan R. Davidson
6. Real-time PCR Quantification of gene Expression in Embryonic Mouse Tissue
Eric Villalon, David J. Schulz and Samuel T. Waters
7. Identifying Essential Genes in Mouse Development Via an ENU-based Forward Genetic Approach
Aimin Liu and Jonathan Eggenschwiler
8. Generation of Mouse Embryos with Small Hairpin RNA-mediated Knockdown of Gene Expression
David A. F. Loebel, Tania Radziewic, Melinda Power, Joshua B. Studdert and Patrick P. L. Tam
9. Generation of Tissue Organoids by Compaction Reaggregation
Julie M. Sheridan and C. Clare Blackburn
10. Ultra-Rapid Vitrification of Mouse Oocytes and Embryos
Mark G. Larman PhD and David K. Gardner
11. Mammalian Preimplantation Embryo Culture
David K. Gardner and Michelle Lane
12. Serum-Free Culture of Mid-Gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived Organs
Julie Gordon, Billie A. Moore, C. Clare Blackburn and
Nancy R. Manley
13. Genetically Encoded Probes Provide a Window On Embryonic Arrhythmia
Yvonne Norine Tallini, Kai Su Greene, Bo Shui, Calum William Russell, Jane Constance Lee, Robert Michael Doran, Junichi Nakai and Michael I. Kotlikoff
14. Microscopic Computed Tomography-based Skeletal Phenotyping for Genetic Model Organisms
Suresh I. Prajapati, Lisa Nevell and Charles Keller
15. Gene Transfer Techniques in Whole Embryo Cultured Post-Implantation Mouse Embryos
Daisuke Sakai and Paul A Trainor
16. Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues
Kaustav Nandy, Jusub Kim, Dean P. McCullough, Matthew McAuliffe, Karen J. Meaburn, Terry P. Yamaguchi, Prabhakar R. Gudla and Stephen J. Lockett
17. Protein/Peptide Transduction in Metanephric Explant Culture
Sergey Plisov, Honghe Wang, Nadya Tarasova, Nirmala Sharma, and Alan O. Perantoni
18. Direction of Cells Programmed to Die in Mouse Embroys
Rocío Hernández-Martínez and Luis Covarrubias
19. Microscopic Computed Tomography-based Virtual Histology of Embryos
Suresh I. Prajapati, David R. Rodriguez, Charles Keller
20. Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study
Yelena Golubeva and David Symer
21. The Latest Improvements in the Mouse Sperm Preservation
Takehito Kaneko
22. Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ in vitro
Satomi S. Tanaka, Yasuka L. Yamaguchi, Vanessa J. Jones and Patrick P. L. Tam
23. Using the Textpresso Site Specific Recombinases Web Server to Identify Cre Expressing Mouse Strains and Floxed Alleles
Brian G. Condie and William M. Urbanski
24. Live Imaging Mouse Embryonic Development: Seeing is Believing and Reveali