1. Growth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse
Stephany El-Hayek, Qin Yang, and Hugh J. Clarke
2. In vitro Culture of Mouse Oocytes for Meiotic Maturation
Ewa Borsuk and Jacek Z. Kubiak
3. In vitro Maturation and Culture of Human Oocytes
Jennifer R Gruhn, Stine Gry Kristensen, Claus Yding Andersen, and Eva R Hoffmann
4. Pronuclear Transfer in Human Oocytes
Louise Hyslop
5. In vitro Fertilization of Mouse Oocyte
Agathe Chaigne
6. Profiling Maternal mRNA Translation during Oocyte Development
Joao P. Sousa Martins and Marco Conti
7. Transcriptome Profiling of Single Mouse Oocytes
Maud Borensztein, Laurène Syx , Nicolas Servant, and Edith Heard
8. Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation
Cecilia S. Blengini and Karen Schindler
9. Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation
Jessica Greaney, Zhe Wei, and Hayden Homer
10. Triple-colour Live Imaging of Mouse Oocytes
Aurélien Courtois, Petr Solc, and Tomoya S. Kitajima
11. Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes
Elvira Nikalayevich, Nora Bouftas, and Katja Wassmann
12. Manipulating Cohesin Levels in Live Mouse Oocytes
Anna Szydlowska, Sabrina Ladstätter, and Kikuë Tachibana
13. Optogenetic Manipulation of Mouse Oocytes
Takashi Akera, David M. Chenoweth, and Michael A. Lampson
14. Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin
Greg FitzHarris
15. Photoactivation of Actin in Mouse Oocyte
Maria Almonacid
16. Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes
Isma Bennabi and Marion Manil-Ségalen
17. Micropipette Aspiration of Oocytes to Assess Cortical Tension
Janice P. Evans and Douglas N. Robinson