1. 50 Years of Myofibroblasts: How the Myofibroblast Concept Evolved
Giulio Gabbiani
Section I: Fundamental Methods to Study Myofibroblast Biology
2. Myofibroblast Functions in Tissue Repair and Fibrosis: An Introduction
Victor J. Thannickal
3. Myofibroblast Markers and Microscopy Detection Methods in Cell Culture and Histology
Fereshteh Younesi, Dong Ok Son, Joao Firmino, and Boris Hinz
4. Fibroblast and Myofibroblast Subtypes: Single Cell Sequencing
Hesham Soliman, Lin Wei Tung, and Fabio M.V. Rossi
5. Myofibroblast Adhesome Analysis by Mass Spectrometry
Christopher A. McCulloch
6. Myofibroblast TGF-β Activation Measurement In Vitro
Joanne Porte, Gisli Jenkins, and Amanda L. Tatler
7. Contraction Measurements Using Three-Dimensional Fibrillar Collagen Gel Lattices
James Griffith and William L. Berry
8. Techniques to Assess Collagen Synthesis, Deposition, and Cross-Linking In Vitro
Tamara Rosell-García and Fernando Rodriguez-Pascual
9. Methods for Studying Myofibroblast Apoptotic Pathways
Yan Zhou and David Lagares
10. Determination of Senescent Myofibroblasts in Precision-Cut Lung Slices
Tamara Cruz, Ana L. Mora, and Mauricio Rojas
11. The Scar-in-a-Jar: In Vitro Fibrosis Model for Anti-Fibrotic Drug Testing
Simon Stebler and Michael Raghunath
Section II: Myofibroblast Mechanobiology
12. Why Stress Matters: An Introduction Daniel J. Tschumperlin
13. Soft Substrate Culture to Mechanically Control Cardiac Myofibroblast Activation
Natalie M. Landry, Sunil G. Rattan, and Ian M.C. Dixon
14. Quantitative Analysis of Myofibroblast Contraction by Traction Force Microscopy
Shuying Yang, Fernando R. Valencia, Benedikt Sabass, and Sergey V. Plotnikov
15. Nucleocytoplasmic Shuttling of the Mechanosensitive Transcription Factors MRTF and YAP/TAZ
Michael Kofler and András Kapus
16. Atomic Force Microscopy for Live-Cell and Hydrogel Measurement
Alexander Whitehead, Natalie J. Kirkland, and Adam J. Engler
17. Method for Investigating Fibroblast Durotaxis
Hossam Kadry, David Lagares, and Taslim A. Al-Hilal
18. Decellularized Extracellular Matrix (ECM) as a Model to Study Fibrotic ECM Mecha
