Part I: Isolation and Analysis of Skeletal Muscle Precursor Cells

1. Isolation and Characterization of Human Fetal Myoblasts

Ariya D. Lapan and Emanuela Gussoni

2. Skeletal Muscle Satellite Cells: Background and Methods for Isolation and Analysis in a Primary Culture System

Maria Elena Danoviz and Zipora Yablonka-Reuveni

3. Isolation of Muscle Stem Cells by Fluorescence Activated Cell Sorting (FACS) Cytometry

Alessandra Pasut, Paul Oleynik, and Michael A. Rudnicki

4. Mouse and Human Mesoangioblasts: Isolation and Characterization from Adult Skeletal Muscles

Mattia Quattrocelli, Giacomo Palazzolo, Ilaria Perini, Stefania Crippa, Marco Cassano, and Maurilio Sampaolesi

5. Direct Electrical Stimulation of Myogenic Cultures for Analysis of Muscle Fiber Type Control

Eric J. Cavanaugh, Jennifer R. Crew, and Joseph X. DiMario

6. Single Muscle-Fiber Isolation and Culture for Cellular, Molecular, Pharmacological, and Evolutionary Studies

Judy E. Anderson, Ashley C. Wozniak, and Wataru Mizunoya

7. Somite Unit Chronometry to Analyze Teratogen Phase Specificity in the Paraxial Mesoderm

Sara J. Venters and Charles P. Ordahl

Part II: Non-Mammalian Models of Myogenesis

8. Analysis of Skeletal Muscle Development in Drosophila

Ginny R. Morriss, Anton L. Bryantsev, Maria Chechenova, Elisa M. LaBeau, TyAnna L. Lovato, Kathryn M. Ryan, and Richard M. Cripps

9. Immunocytochemistry to Study Myogenesis in Zebrafish

Nathan C. Bird, Stefanie E. Windner, and Stephen H. Devoto

10. Immunofluorescent Localization of Proteins in C. elegans Muscle

Kristy J. Wilson, Hiroshi Qadota, and Guy M. Benian

Part III: Experimental Models and Analysis of Skeletal Muscle Exercise and Disuse

11. Resistance Loading and Signaling Assays for Oxidative Stress in Rodent Skeletal Muscle

Stephen E. Alway and Robert G. Cutlip

12. Analysis of Skeletal Muscle Hypertrophy in Models of Increased Loading

Sue C. Bodine and Keith Baar

13. Protein Overexpression in Skeletal Muscle Using Plasmid-Based Gene Transfer to Elucidate Mechanisms Controlling Fiber Size

Chia-Ling Wu and Susan C. Kandarian

14. In Vivo Measurement of Muscle Protein Synthesis Rate Using the Flooding Dose Technique

Marta L. Fiorotto, Horacio A. Sosa, Jr., and Teresa A. Davis

Part IV: Generation of Viral Vectors and Transgenic Mice

15. Recombinant Adeno-Associated Viral Vector Production and Purification

Jin-Hong Shin, Yongping Yue, and Dongsheng Duan

16. Generation of Lentiviral Vectors for Use in Skeletal Muscle Research

Christophe Pichavant and Jacques P. Tremblay

17. Generating Tamoxifen-Inducible Cre Alleles to Investigate Myogenesis in Mice

Christoph Lepper and Chen-Ming Fan

Part V: Muscle Profiling

18. Gene Profiling Studies in Skeletal Muscle by Quantitative Real-Time Polymerase Chain Reaction Assay

Shephali Bhatnagar, Siva K. Panguluri, and Ashok Kumar

19. Analysis of Lipid Profiles in Skeletal Muscles

Vassilis Mougios and Anatoli Petridou

20. Proteomic Analysis of Dystrophic Muscle

Caroline Lewis, Philip Doran, and Kay Ohlendieck