1. Introduction: Nanoimaging Techniques in Biology

Alioscka A. Sousa and Michael J. Kruhlak

Part I. Light Microscopy

2. Live-Cell Imaging of Vesicle Trafficking and Divalent Metal Ions by Total Internal Reflection Fluorescence (TIRF) Microscopy

Merewyn K. Loder, Takashi Tsuboi, and Guy A. Rutter

3. 4Pi Microscopy

Roman Schmidt, Johann Engelhardt, and Marion Lang

4. Fluorescence in situ Hybridization Applications for Super-Resolution 3D Structured Illumination Microscopy

Yolanda Markaki, Daniel Smeets, Marion Cremer, and Lothar Schermelleh

5. Two-Color STED Imaging of Synapses in Living Brain Slices

Jan Tønnesen, and U. Valentin Nägerl

6. Super-Resolution Imaging by Localization Microscopy

Dylan M. Owen, Astrid Magenau, David Williamson, and Katharina Gaus

7. High-Content Super-Resolution Imaging of Live Cell by uPAINT

Grégory Giannone, Eric Hosy, Jean-Baptiste Sibarita, Daniel Choquet, and Laurent Cognet

8. Super-Resolution Fluorescence Imaging with Blink Microscopy

Christian Steinhauer, Michelle S. Itano, and Philip Tinnefeld

9. Photoswitchable Fluorophores for Single-Molecule Localization Microscopy

Kieran Finan, Benjamin Flottmann, and Mike Heilemann

10. Single-Molecule Tracking of mRNA in Living Cells

Mai Yamagishi, Yoshitaka Shirasaki, and Takashi Funatsu

Part II. Electron Microscopy

11. Semiautomatic, High-Throughput, High-Resolution Protocol for Three-Dimensional Reconstruction of Single Particles in Electron Microscopy

Carlos Oscar Sorzano, J. M. de la Rosa Trevín, J. Otón, J. J. Vega, J. Cuenca, A. Zaldívar-Peraza, J. Gómez-Blanco, J. Vargas, A. Quintana, R. Marabini, and J. M. Carazo

12. Mass Mapping of Amyloid Fibrils in the Electron Microscope using STEM Imaging

Alioscka A. Sousa and Richard D. Leapman

13. Elemental Mapping by Electron Energy Loss Spectroscopy in Biology

Maria A. Aronova and Richard D. Leapman

14. Cellular Nanoimaging by Cryo Electron Tomography

Roman I. Koning and Abraham J. Koster

15. Large-Volume Reconstruction of Brain Tissue from High-Resolution Serial Section Images Acquired by SEM-Based Scanning Transmission Electron Microscopy

Masaaki Kuwajima, John M. Mendenhall, and Kristen M. Harris

16. 3D Imaging of Cells and Tissues by Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM)

Damjana Drobne

17. Preparation of Gold Nanocluster Bioconjugates for Electron Microscopy

Christine L. Heinecke and Christopher J. Ackerson

Part III. Scanning Probe Microscopy

18. Atomic Force Microscopy Imaging of Macromolecular Complexes

S. Santos, D.J. Billingsley, and N.H. Thomson

19. Imaging of Transmembrane Proteins Directly Incorporated Within Supported Lipid Bilayers Using Atomic Force Microscopy

Daniel Levy and Pierre-Emmanuel Milhiet

20. Functional AFM Imaging of Cellular Membranes Using Functionalized Tips

Lilia A. Chtcheglova and Peter Hinterdorfer

21. Near-Field Scanning Optical Microscopy for High Resolution Membrane Studies

Heath A. Huckabay, Kevin P. Armendariz, William H. Newhart, Sarah M. Wildgen, and Robert C. Dunn

Part IV. Complementary Techniques

22. Correlative Fluorescence and EFTEM Imaging of the Organized Components of the Mammalian Nucleus

Michael J. Kruhlak

23. High Data Output Method for 3-D Correlative Light-Electron Microscopy Using Ultrathin Cryosections

Katia Cortese, Giuseppe Vicidomini, Maria C. Gagliani, Patrizia Boccacci, Alberto Diaspro, and Carlo Tacchetti

24. Correlative Optical and Scanning Probe Micr