Part I: Preliminary Sample Preparation

1. MALDI-TOF Serum Profiling Using Semi-Automated Serum Peptide Capture with Magnetic Reversed Phase (C18) Beads

Jaco C. Knol and Connie R. Jimenez

2. Proteomics of Epithelial Lining Fluid Obtained by Bronchoscopic Microprobe Sampling

Lorenza Franciosi, Natalia Govorukhina, Nick ten Hacken, Dirkje Postma, and Rainer Bischoff

Part II: Nanoscale Separation

3. Protein Identification Using Nano-HPLC-MS: ESI-MS and MALDI-MS Interfaces

Rui Vitorino, Jana Krenkova, Frantisek Foret, Pedro Domingues, and Francisco Amado

4. Three Dimensional Peptide Fractionation for Highly Sensitive Nanoscale LC-Based Shotgun Proteomic Analysis of Complex Protein Mixtures

Sricharan Bandhakavi, Todd W. Markowski, Hongwei Xie, and Timothy J. Griffin

5. Nanospray Ion Mobility Mass Spectrometry of Selected High Mass Species

Iain Campuzano and Kevin Giles

6. Nanoelectrospray-MSⁿ of Native and Permethylated Glycans

Christina Bleckmann, Hildegard Geyer, and Rudolf Geyer

7. N-linked Global Glycan Profiling by NanoLC Mass Spectrometry

Michael S. Bereman and David C. Muddiman

8. Applications of Nanoscale Liquid Chromatography Coupled to Tandem Mass Spectrometry in Quantitative Studies of Protein Expression, Protein-Protein Interaction, and Protein Phosphorylation

Metodi V. Metodiev

9. Nano LC-MS/MS: A Robust Setup for Proteomic Analysis

Marco Gaspari and Giovanni Cuda

10. Nanofluidic Devices for Rapid Continuous-Flow Bioseparation

Pan Mao and Jianping Fu

11. Quantifying Attomole Amounts of Proteins from Complex Samples by Nano-LC and Selected Reaction Monitoring (SRM)

Thomas Fröhlich and Georg J. Arnold

Part III: Nanomaterials: Nanostructured Surfaces

12. A Sample Preparation Method for Gold Nanoparticle-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Yen-Hsiu Lin and Wei-Lung Tseng

13. Nanostructured TiO₂ Thin Films for Phosphoproteomics Studies with MALDI Mass Spectrometry

Federico Torta, Matteo Fusi, Carlo S.Casari, Andrea Li Bassi, and Angela Bachi

14. Nanofilament Silicon for Matrix-Free Laser Desorption/Ionization Mass Spectrometry

Chia-Wen Tsao and Don L. DeVoe

15. Protein Nanoarrays for High Resolution Patterning of Bacteria on Gold Surfaces

Cait Costello, Jan-Ulrich Kreft, Christopher M. Thomas, and Paula M. Mendes

Part IV: Nanomaterials: Nanoscale Tools for Proteomic Discovery

16. Engineered Multifunctional Nanotools for Biological Applications

Mohammed Ibrahim Shukoor, Muhammad Nawaz Tahir, Thomas Schladt, Wolfgang Tremel, Zhiqun Zhang, Kevin K. Wang, and Firas Kobeissy

17. The Selective Capture of Phosphopeptides by Zirconium Phosphonate-Modified Magnetic Nanoparticles

Liang Zhao, Ren'an Wu, and Hanfa Zou

18. Nanowire Biosensors for Label-Free, Real-Time, Ultrasensitive Protein Detection

Gengfeng Zheng and Charles M. Lieber

19. Generation of Anti-Infectome/Anti-Proteome Nanobodies

Gholamreza Hassanzadeh-Ghassabeh, Dirk Saerens, and Serge Muyldermans

Part V: Nanotechnology: Techniq