Nanoscale Imaging

Preface...

Table of Contents...

Contributing Authors...

Part I Imaging and Probing of Biomolecules

1. High Resolution Atomic Force Microscopy Imaging of Nucleic Acids

Pablo Ares, Julio Gomez-Herrero, and Fernando Moreno-Herrero

2. Two Ligand Binding Sites in Serotonin Transporter Revealed by Nanopharmacological Force Sensing

Rong Zhu, Hermann J. Gruber, and Peter Hinterdorfer

3. AFM-Based Single-Molecule Force-Spectroscopy of Proteins

Zackary N. Scholl and Piotr E. Marszalek

4. High Resolution AFM-Based Force Spectroscopy

Krishna P. Sigdel, Anna E. Pittman, Tina R. Matin, and Gavin M. King

5. Polymer Nanoarray Approach for the Characterization of Biomolecular Interactions

Sibaprasad Maity, Ekaterina Viazovkina, Alexander Gall, and Yuri L. Lyubchenko

6. Measuring Single-Molecule Twist and Torque in Multiplexed Magnetic Tweezers

Franziska Kriegel, Willem Vanderlinden, Thomas Nicolaus, Angelika Kardinal, and Jan Lipfert

7. AFM-Based Characterization of Electrical Properties of Materials

John Alexander, Sergey Belikov, and Sergei Magonov

8. Supported Lipid Bilayers for Atomic Force Microscopy Studies

Zhengjian Lv, Siddhartha Banerjee, Karen Zagorski, and Yuri L. Lyubchenko

9. Quantifying Small Molecule Binding Interactions with DNA Nanostructures

Xuye Lang, Yingning Gao, and Ian Wheeldon

Part II High-Speed AFM of Biomolecules

10. Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy

Takayuki Uchihashi, Hiroki Watanabe, and Noriyuki Kodera

11. High Resolution and High-Speed Atomic Force Microscope Imaging

Francesca Zuttion, Lorena Redondo-Morata, Arin Marchesi, and Ignacio Casuso

12. High-Speed Atomic Force Microscopy of Individual Amyloidogenic Protein Assemblies

Takahiro Watanabe-Nakayama and Kenjiro Ono

13. Direct Observation of Dynamic Movement of DNA Molecules in DNA Origami Imaged using High-Speed AFM

14. Assembly of Centromere Chromatin for Characterization By High-Speed Time-Lapse Atomic Force Microscopy

Micah P. Stumme-Diers, Siddhartha Banerjee, Zhiqiang Sun, and Yuri L. Lyubchenko

15. High-Speed Force Spectroscopy for Single Protein Unfolding

Part III Imaging and Probing of Biomolecular Complexes

16. Probing RNA-Protein Interactions with Single-Molecule Pull-Down Assays

Mohamed Fareh and Chirlmin Joo

17. Preparing Frozen-Hydrated Protein-Nucleic Acid Assemblies for High-Resolution Cryo-EM Imaging

Panchali Goswami, Julia Locke, and Alessandro Costa

18. Probing Chromatin Structure with Magnetic Tweezers

Artur Kaczmarczyk, Thomas B. Brouwer, Chi Pham, Nynke H. Dekker, and John van Noort

Jaya Sarkar and Sua Myong

20. Correlative Atomic Force and Single Molecule Fluorescence Microscopy of Nucleoprotein Complexes

Herlinde De Keersmaecker, Wout Frederickx, Yasuhiko Fujita; Steven De Feyter, Hiroshi Uji-i, Susana Rocha, and Willem Vanderlinden

Part IV Imaging and Probing of Cells and Tissues and Embryos

21. Sensing the Ultrastructure of Bacterial Surfaces and their Molecular Binding Forces using AFM

Yoo Jin Oh and Peter Hinterdorfer

22. Nanoscale Visualization of Bacterial Microcompartments using Atomic Force Microscopy

Jorge Rodriguez-Ramos, Matthew Faulkner, and Lu-Ning Liu

23. Time-Resol