Part I: NK Cell Specification

1. Isolation of Innate Lymphoid Cells from Murine Intestinal Lamina Propria

Ao Mei, Elaheh Hashemi, Mohamed Khalil, Dandan Wang, and Subramaniam Malarkannan

2. Purification of Primary Decidual Natural Killer Cells for Functional Analysis

Ângela C. Crespo, Aria Alexander, and Tamara Tilburgs

3. In Vitro Development of Mouse and Human NK Cells from Hematopoietic Progenitor Cells

Ines Ullmo, Nahide Koksal, Heather Y.K. Ang, and Hugh J.M. Brady

4. Induction of Human Natural Killer Cells under Defined Conditions by Seamless Transition from Maintenance Culture of Pluripotent Stem Cells

Akira Niwa and Megumu K. Saito

5. Development of Humanized Mouse Models for Studying Human NK Cells in Health and Disease

Liang Shan, Richard A. Flavell, and Dietmar Herndler-Brandstetter

6. Antibody-Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10x Genomics' Single-Cell RNA-Seq

Dominic Paul Lee, Wang Jiehao Ray, Tan Pee Mei, Shawn Hoon, Jonathan Scolnick, and Gene W. Yeo

7. Methods to Analyze the Developmental Trajectory of Human Primary NK Cells Using Monocle and SCENIC Analyses

Dandan Wang, Robert Burns, Mohamed Khalil, Ao Mei, Elaheh Hashemi, and Subramaniam Malarkannan

8. Methods for Isolating and Defining Single-Cell Transcriptomes of Tissue-Resident Human NK Cells

Elaheh Hashemi, Ao Mei, Dandan Wang, Mohamed Khalil, and Subramaniam Malarkannan

9. Retrogenic Color-Barcoding for Fate Mapping of Single Innate Lymphocytes

Simon Grassmann, Joseph C. Sun, and Veit R. Buchholz

10. Quantifying Human Natural Killer Cell Migration by Imaging and Image Analysis

Amera L. Martinez, Michael J. Shannon, Shira E. Eisman, Everardo Hegewisch-Solloa, Aneeza N. Asif, Tasneem A.M. Ebrahim, and Emily M. Mace

11. Tracking of NK Cells by Positron Emission Tomography Using ⁸⁹Zr-Oxine Ex Vivo Cell Labeling

Noriko Sato, Lawrence P. Szajek, and Peter L. Choyke

Part II: NK Cell Functional Assays

12. Assessing Changes in Human Natural Killer Cell Metabolism Using the Seahorse Extracellular Flux Analyzer

Javier Traba and Olga M. Antón

13. Determining Activation Status of Natural Killer Cells Following Stimulation via Cytokines and Surface Receptors

Lizeth G. Meza Guzman and Sandra E. Nicholson

14. Method to Study Adaptive NK Cells Following MCMV Infections

Mohamed Khalil, Ao Mei, Elaheh Hashemi, Dandan Wang, Megan Schumacher, Scott Terhune, and Subramaniam Malarkannan

15. Assessing the Response of Human NK Cell Subsets to Infec