1. An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate in vivo Translatome
Hyun Yong Jin and Changchun Xiao
2. Measuring Nascent Transcripts by Nascent-seq
Fei Xavier Chen, Stacy A. Marshall, and Yu Deng
3. Genome-wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis
Lieselot Deleye, Dieter De Coninck, Dieter Deforce, and Filip Van Nieuwerburgh
4. Multiplexed Targeted Sequencing for Oxford Nanopore MinION; A Detailed Library Preparation Procedure
Timokratis Karamitros and Gkikas Magiorkinis
5. Hi-Plex for Simple, Accurate, and Cost-effective Amplicon-based Targeted DNA Sequencing Bernard J Pope, Fleur Hammet, Tu Nguyen-Dumont, and Daniel J Park
6. ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras
Elizabeth Jaworski and Andrew Routh
7. Genome-wide Analysis of DNA Methylation in Single Cells Using a Post-bisulfite Adapter Tagging Approach
Heather J Lee and Sébastien A Smallwood
8. Sequencing of Genomes from Environmental Single Cells
Robert M. Bowers, Janey Lee, and Tanja Woyke
9. SNP Discovery from Single and Multiplex Genome Assemblies of Non-model Organisms
Phillip A. Morin, Andrew D. Foote, Christopher M. Hill, Benoit Simon-Bouhet, Aimee R. Lang, and Marie Louis
10. CleanTag Adapters Improve Small RNA Next-Generation Sequencing Library Preparation by Reducing Adapter Dimers
Sabrina Shore, Jordana M. Henderson, and Anton P. McCaffrey
11. Sampling, Extraction, and High-Throughput Sequencing Methods for Environmental Microbial and Viral Communities Pedro J. Torres and Scott T. Kelley
12. A Bloody Primer Analysis of RNA-Seq from Tissue Admixtures
Casey P. Shannon, Chen Xi Yang, and Scott J. Tebbutt
13. Next-Generation Sequencing of Genome-wide CRISPR Screens
Edwin Yau and Tariq Rana
14. Gene Profiling and T Cell Receptor Sequencing from Antigen-specific CD4 T Cells
Marie Holt, Anne Costanzo, Louis Gioia, Brian Abe, Andrew I. Su, and Luc Teyton
15. Investigate Global Chromosomal Interaction by Hi-C in Human Naïve CD4 T Cells
Xiangzhi Meng, Nicole Riley, Ryan Thompson, and Siddhartha Sharma
16. Primer Extension, Capture, and On-bead cDNA Ligation: An Efficient RNAseq Library Prep Method for Determining Reverse Transcription Termination Sites
Phillip Ordoukhanian, Jessica Nichols, and Steven R Head
