Part I: Background and Overview

1. Structural Biology of Nonribosomal Peptide Synthetases

Bradley R. Miller and Andrew M. Gulick

2. The Assembly Line Enzymology of Polyketide Biosynthesis

Marisa Till and Paul R. Race

Part II: In Vitro Methods

3. Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydroxylamine Release Assay

Benjamin P. Duckworth, Daniel J. Wilson, and Courtney C. Aldrich

4. Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains

Fumihiro Ishikawa and Hideaki Kakeya

5. Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetases

Chitose Maruyama, Haruka Niikura, Masahiro Takakuwa, Hajime Katano, and Yoshimitsu Hamano

6. Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis

Clara Brieke, Veronika Kratzig, Madeleine Peschke, and Max J. Cryle

7. Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In Vitro

Ralph A. Cacho and Yi Tang

8. The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis

Haoran Zhang, Lei Fang, Marcia S. Osburne, and Blaine A. Pfeifer

9. Screening for Expressed Non

Yunqiu Chen, Ryan A. McClure, and Neil L. Kelleher

10. Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi

Alexandra A. Soukup, Nancy P. Keller, and Philipp Wiemann

11. In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Delphine Debois, Marc Ongena, Hélène Cawoy, and Edwin De Pauw

12. Secondary Metabolic Pathway-Targeted Metabolomics

Maria I. Vizcaino and Jason M. Crawford

Part III: Bioinformatic Methods

13. Annotating and Interpreting Linear and Cyclic Peptide Tandem Mass Spectra

14. Bioinformatics Tools for the Discovery of New Nonribosomal Peptides

Valérie Leclère, Tilmann Weber, Philippe Jacques, and Maude Pupin

15. The Use of ClusterMine360 for the Analysis of Polyketide and Nonribosomal Peptide Biosynthetic Pathways

Nicolas Tremblay, Patrick Hill, Kyle R. Conway, and Christopher N. Boddy

16. Alignment-Free Methods for the Detection and Specificity Prediction of Adenylation Domains

Guillermin Agüero-Chapin, Gisselle Pérez-Machado, Aminael Sánchez-Rodríguez, Miguel Machado Santos, and Agostinho Antunes

17. Characterization of Nonribosomal Peptide Synthetases with NRPSsp

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