1. Introduction to Notch Signaling
Shinya Yamamoto, Karen L. Schulze, and Hugo J. Bellen
2. Genetic Screens to Identify New Notch Pathway Mutants in Drosophila
Nikolaos Giagtzoglou
3. Structure-function Analysis of Drosophila Notch using Genomic Rescue Transgenes
Jessica Leonardi and Hamed Jafar-Nejad
4. Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice
Thomas Gridley and Andrew K. Groves
5. Immunohistochemical Tools and Techniques to Visualize Notch in Drosophila melanogaster
Emiliana Tognon and Thomas Vaccari
6. Antibody Uptake Assay and in vivo Imaging to Study Intracellular Trafficking of Notch and Delta in Drosophila
Lydie Couturier and François Schweisguth
7. Tracking Trafficking of Notch and its Ligands in Mammalian Cells
Patricia Chastagner and Christel Brou
8. Visualizing Notch Signaling in vivo in Drosophila Tissues
Benjamin E. Housden, Jinghua Li, and Sarah J. Bray
9. Monitoring Notch Activity in the Mouse
Lavinia Alberi and Swananda Marathe
10. Notch Signaling Assays in Drosophila Cultured Cell Lines
Jinghua Li, Benjamin E. Housden, and Sarah J. Bray
11. Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter Assays
Ma. Xenia G. Ilagan and Raphael Kopan
12. Monitoring Notch Activation in Cultured Mammalian Cells: Luciferase Complementation Imaging Assays
Ma. Xenia G. Ilagan and Raphael Kopan
13. Visualization of Notch Signaling Oscillation in Cells and Tissues
Hiromi Shimojo, Yukiko Harima, and Ryoichiro Kageyama
14. Proteomic Analysis of the Notch Interactome
K. G. Guruharsha, Kazuya Hori, Robert A. Obar, and Spyros Artavanis-Tsakonas
15. Bacterial Expression and in vitro Refolding of Limited Fragments of the Notch Receptor and its Ligands
Pat Whiteman, Christina Redfield and Penny A. Handford
16. Analyzing the Post-translational Modification Status of Notch using Mass Spectrometry
Shinako Kakuda and Robert S. Haltiwanger
17. Assay to Probe Proteolytic Processing of Notch by γ-secretase
Lutgarde Serneels, Ina Tesseur and Bart De Strooper
18. Analyzing the Nuclear Complexes of Notch Signaling by Electrophoretic Mobility Shift Assay
Kelly L. Arnett and Stephen C. Blacklow
19. Identifying Direct Notch Transcriptional Targets using the GSI-washout Assay
Will Bailis, Yumi Yashiro-Ohtani and Warren S. Pear
20. Probing the Epigenetic Status at Notch Target Genes Tilman Borggrefe and Robert Liefke
21. Notch-ligand Binding Assays in Drosophila Cells
Aiguo Xu and Kenneth D. Irvine
22. Modeling Notch Signaling: A Practical Tutorial
Pau Formosa-Jordan and David Sprinzak
23. Small Molecules that Inhibit Notch Signaling
Gerdien E. De Kloe and Bart De Strooper
24. Application and Evaluation of Anti-Notch Antibodies to Modulate Notch Signaling
Wendy R. Gordon and Jon C. Aster
25. Application of Anti-ligand Antibodies to Inhibit Notch Signaling
Jun-ichiro Koga and Masanori Aikawa
