1. Histological Analysis of the Embryonic and Adult Tooth
Atsushi Ohazama
2. Determination of Gene Expression Patterns by Whole-Mount In Situ Hybridization
Sergiy Kyryachenko, Kateryna Kyrylkova, Mark Leid, and Chrissa Kioussi
3. Determination of Gene Expression Patterns by In Situ Hybridization in Sections
Kateryna Kyrylkova, Sergiy Kyryachenko, Chrissa Kioussi, and Mark Leid
4. Immunohistochemistry and Detection of Proliferating Cells by BrdU
Sergiy Kyryachenko, Kateryna Kyrylkova, Mark Leid, and Chrissa Kioussi
5. Detection of Apoptosis by TUNEL Assay
Kateryna Kyrylkova, Sergiy Kyryachenko, Mark Leid, and Chrissa Kioussi
6. Use of siRNA in Dental Tissue-Derived Cell Cultures: Integrin Knockdown in Fibroblasts
Malgorzata M. Barczyk, Donald Gullberg, and Anne Isine Bolstad
7. Organ Cultures and Kidney-Capsule Grafting of Tooth Germs
Keishi Otsu, Naoki Fujiwara, and Hidemitsu Harada
8. Evaluation of Skull and Tooth Morphology and Mineralization Using High-Resolution X-Ray Tomography
Brian K. Bay
9. Electron Microscopy
Hans U. Luder and Margrit Amstad-Jossi
10. Deoxyoligonucleotide Microarrays for Gene Expression Profiling in Murine Tooth Germs
Harald Osmundsen, Anne-Marthe Jevnaker, and Maria A. Landin
11. Lineage Differentiation of Mesenchymal Stem Cells from Dental Pulp, Apical Papilla, and Periodontal Ligament
Kentaro Akiyama, Chider Chen, Stan Gronthos, and Songtao Shi
12. In Vivo Transplantation and Tooth Repair
Shuhei Tsuchiya and Masaki Honda
13. Methods to Validate Tooth-Supporting Regenerative Therapies
Miguel Padial-Molina, Julie T. Marchesan, Andrei D. Taut, Qiming Jin, William V. Giannobile, and Hector F. Rios
14. Generation of a Bioengineered Tooth by Using a Three-Dimensional Cell Manipulation Method (Organ Germ Method)
Masamitsu Oshima, Miho Ogawa, Masato Yasukawa, and Takashi Tsuji
15. In Vitro Studies on Odontogenic Tumors
Javier Catón, Thimios A. Mitsiadis, and Peter R. Morgan
16. Whole Mount Immunohistochemistry and In Situ Hybridization of Larval and Adult Zebrafish Dental Tissues
Barbara Verstraeten, Ellen Sanders, and Ann Huysseune