Part I: Fundamentals of Using Light to Study Neocortical Dynamics
1. Neocortex in the Spotlight: Concepts, Questions, and Methods
Fritjof Helmchen and Bruno Weber
2. Principles and Fundamentals of Optical Imaging
Frank Scheffold
3. Optical Properties of Neural Tissue
Andrew K. Dunn
4. Fluorescent Indicators for Functional Optical Imaging
Walther Akemann, Hiroki Mutoh, and Thomas Knöpfel
5. Optogenetic Tools for Control of Neural Activity
Lief E. Fenno and Karl Deisseroth
Part II: Shedding Light on Cortical Activity States
6. In Vivo Dendritic Mapping of Sensory Inputs in Cortical Neurons
Hongbo Jia and Arthur Konnerth
7. In Vivo Population Imaging of Dendritic Integration in Neocortex
Masanori Murayama and Matthew E. Larkum
8. Voltage-Sensitive Dye Imaging of Cortical Dynamics
Carl C.H. Petersen
9. Two-Photon Imaging of Neuronal Network Dynamics in Neocortex
Benjamin F. Grewe, Fritjof Helmchen, and Björn M. Kampa
10. Chronic Two-Photon Imaging of Neural Activity in the Anesthetized and Awake Behaving Rodent
David J. Margolis, Henry Lütcke, Fritjof Helmchen, Bruno Weber, and Florent Haiss
11. Neocortical Circuit Interrogation with Optogenetics
Lief E. Fenno and Karl Deisseroth
Part III: Elucidating Metabolic States of Cortical Circuits
12. Imaging of Astrocytic Activity in Living Rodents
Norio Takata, Yoshiaki Shinohara, Masamichi Ohkura, Tsuneko Mishima, Junichi Nakai, and Hajime Hirase
13. Imaging Morphology and Function of Cortical Microglia
Bianca Brawek and Olga Garaschuk
14. Functional Imaging of Cerebral Oxygenation with Intrinsic Optical Contrast and Phosphorescent Probes
Anna Devor, Sava Sakadzic, Mohammad A. Yaseen, Emmanuel Roussakis, Peifang Tian, Hamutal Slovin, Ivo Vanzetta, Ivan Teng, Payam A. Saisan, Louise E. Sinks, Anders M. Dale, Sergei A. Vinogradov, and David A. Boas
15. Laser Speckle Imaging of Cerebral Blood Flow
Pavel Zakharov, Frank Scheffold, and Bruno Weber
16. Two-Photon Microscopy to Measure Blood Flow and Concurrent Brain Cell Activity
Andy Y. Shih, Jonathan D. Driscoll, Michael J. Pesavento, and David Kleinfeld