About the Book
Preface...
Table of Contents...
Contributing Authors...
Part I Saliva and Other Oral Fluids
1. Salivary Diagnostics using Purified Nucleic Acids
Paul D. Slowey
2. RNA Sequencing Analysis of Salivary Extracellular RNA
Blanca Majem, Feng Li, Jie Sun, and David T.W. Wong
3. Qualitative and Quantitative Proteomic Analysis of Oral Fluids in Health and Periodontal Disease by Mass Spectrometry
Erdjan Salih
4. Antioxidant Micronutrients and Oxidative Stress Biomarkers
Iain L.C. Chapple, Helen R. Griffiths, Mike R. Milward, Martin R. Ling, and Melissa M. Grant
5. NMR-Based Metabolomics of Oral Biofluids
Horst Joachim Schirra and Pauline J. Ford
6. Gene Therapy of Salivary Diseases
Bruce J. Baum, Sandra Afione, John a. Chiorini, Ana P. Cotrim, Corinne M. Goldsmith, and Changyu Zheng
Part II Molecular Biosciences
7. The Oral Microbiota in Health and Disease: An Overview of Molecular Findings
José F. Siqueira, Jr., and Isabela N. Rôças
8. Microbial Community Profiling using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)
José F. Siqueira, Jr., Mitsuo Sakamoto, and Alexandre S. Rosado
9. Analysis of 16S rRNA Gene Amplicon Sequences using the QIIME Software Package
Blair Lawley and Gerald W. Tannock
10. Adhesion of Yeast and Bacteria to Oral Surfaces
Richard D. Cannon, Karl M. Lyons, Kenneth Chong, Kathryn Newsham-West, Kyoko Niimi, and Ann R. Holmes
11. Quantitative Analysis of Periodontal Pathogens by Real-Time Polymerase Chain Reaction
Ma José Marin, Elena Figuero, David Herrera, and Mariano Sanz
12. Methods to Study Antagonistic Activities Among Oral Bacteria
Fengxia Qi and Jens Kreth
13. Natural Transformation of Oral Streptococci by Use of Synthetic Pheromones Gabriela Salvadori, Roger Junges, Rabia Khan, Heidi A. Åmdal, Donald Morrison, and Fernanda C. Petersen
14. Markerless Genome Editing in Competent Streptococci
Roger Junges, Rabia Khan, Yanina Tovpeko, Heidi A. Åmdal, Fernanda C. Petersen, and Donald A. Morrison
15. Tools and Strategies for Analysis of Genome-Wide and Gene-Specific DNA Methylation Patterns
Aniruddha Chatterjee, Euan J. Rodger, Ian M. Morison, Michael R. Eccles, and Peter A. Stockwell
16. Generating Multiple Base-Resolution DNA Methylomes using Reduced Representation Bisulfite Sequencing
Aniruddha Chatterjee, Euan J. Rodger, Peter a. Stockwell, Gwenn Le Mée, and Ian M. Morison
17. A Protocol for the Determination of the Methylation Status of Gingival Tissue DNA at Specific CpG Islands
Trudy J. Milne
18. Genome-Wide Expression Analysis of Periodontal Cells and Tissues
Moritz Kebschull, Claudia Hülsmann, Per Hoffmann, and Panos N. Papapanou
19. Differential Expression and Functional Analysis of High-Throughput -Omics Data Using Open Source Tools
Moritz Kebschull, Julia M. Fittler, Ryan T. Demmer, and Panos N. Papapanou
20. Exploring Genome-Wide Expression Profiles Using Machine Learning Techniques
Moritz Kebschull and Panos N. Papapanou
Part III Cells and Tissues
21. Embryonic Explant Culture: Studying Effects of Regulatory Molecules on Gene Expression in Craniofacial Tissues
Katja Närhi
22. Oral Epithelial Cell Culture Model for Studying the Pathogenesis of Chronic Inflammatory Disease
Mike R. Milward, Martin R. Ling, Melissa M. Grant, Iain L.C. Chapple
23. Fabrication and Characterization of Decellularized Periodontal Ligament Cell Sheet Constructs
Amro Farag, Cedryck Vaquette, Dietmar W. Hutmacher, P. Mark Bartold, and Saso Ivanovski
24. A Method to Isolate, Purify and Characterize Human Periodontal Ligament Stem Cells
