1. Detecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues
Marie P. Khoury, Virginie Marcel, Kenneth Fernandes, Alexandra Diot, David P. Lane and Jean-Christophe Bourdon
2. Detecting p53 Isoforms at Protein Level
Virginie Marcel, Marie P. Khoury, Kenneth Fernandes, Alexandra Diot, David P. Lane and Jean-Christophe Bourdon
3. Autophagy, Senescence and Apoptosis
Rachel W. Goehe, Molly L. Bristol, Eden N. Wilson, and David A. Gewirtz
4. p53 and Cell Cycle Effects after DNA Damage
Emir Senturk, James J. Manfredi
5. p53 Ubiquitination and Proteasomal Degradation
Ian Love, Dingding Shi, Steven R. Grossman
6. Identification of p53 in Mitochondria
Angelina V. Vaseva and Ute M. Moll
7. Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis
Sumitra Deb and Paul R. Graves
8. Identification of Small Molecules Affecting p53-MDM2/MDMX Interaction by Fluorescence Polarization
Qi Zhang, and Hua Lu
9. Determine the Effect of p53 on Chemosensitivity
Emir Senturk, James J. Manfredi
10. Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells
Mahesh Ramamoorthy, Catherine Vaughan, Sumitra Deb, Swati Palit Deb
11. Mutant p53 in Cell Adhesion and Motility
W. Andrew Yeudall, Katharine H. Wrighton, and Sumitra Deb
12. Use of the DNA Fiber Spreading Technique to Detect the Effects of Mutant p53 on DNA Replication
Rebecca A. Frum, Sumitra Deb and Swati Palit Deb
13. Generation of p53-deficient Induced Pluripotent Stem Cells (iPS) from Mouse Embryo Fibroblasts
Evguenia M. Alexandrova and Ute M. Moll
14. p53 Actions on MicroRNA Expression and Maturation Pathway
Hiroshi I. Suzuki and Kohei Miyazono
15. Isolation and Characterization of Murine Multipotent Lung Stem Cells
Venkat S Gadepalli, Catherine A Vaughan, Raj R. Rao
16. Generation of p53 Knock-down Cell Lines
Catherine Vaughan, Swati Palit Deb, Sumitra Deb
17. ChIP for Identification of p53 Responsive DNA Promoters
Jun-Ming Liao and Hua Lu
18. ChIP-on-chip to Identify Mutant p53 Targets
Frauke Goeman, Giulia Fontemaggi, and Giovanni Blandino
19. ChIP Sequencing to Identify p53 Targets
Catherine Vaughan, Brad Windle, Sumitra Deb
20. The p53-Mdm2 Loop: A Critical Juncture of Stress Response
Yaara Levav-Cohen, Zehavit Goldberg, Valentina Zuckerman, Sue Haupt, and Ygal Haupt