Part I: Peptide Arrays: Cutting-Edge Methods and Technology
1. Peptide Arrays on Planar Supports
Víctor Tapia and Rudolf Volkmer
2. High-Throughput Microarray Incubations Using Multi-Well Chambers
Johannes Zerweck, Ulf Reimer, Janina Jansong, Nikolaus Pawlowski, Christoph Tersch, Maren Eckey, and Tobias Knaute
3. Analysis of Protein Tyrosine Kinase Specificity Using Positional Scanning Peptide Microarrays
Yang Deng and Benjamin E. Turk
4. Secondary Structure Determination of Peptides and Proteins After Immobilization
Stella H. North and Chris R. Taitt
5. Peptides and Anti-Peptide Antibodies for Small and Medium Scale Peptide and Anti-Peptide Affinity Microarrays: Antigenic Peptide Selection, Immobilization, and Processing
Fan Zhang, Andrea Briones, and Mikhail Soloviev
6. Low-Cost Peptide Microarrays for Mapping Continuous Antibody Epitopes
Ryan McBride, Steven R. Head, Phillip Ordoukhanian, and Mansun Law
7. The Peptide Microarray-Based Resonance Light Scattering Assay for Sensitively Detecting Intracellular Kinase Activity
Tao Li, Xia Liu, Dianjun Liu, and Zhenxin Wang
8. Anomalous Reflection of Gold: A Novel Platform for Biochips
Amir Syahir, Kin-ya Tomizaki, Kotaro Kajikawa, and Hisakazu Mihara
9. High Throughput Peptide Screening on a Bimodal Imprinting Chip Through
MS-SPRi Integration Weizhi Wang, Qiaojun Fang, and Zhiyuan Hu
10. Analyzing Peptide Microarray Data with the R pepStat Package
Gregory Imholte, Renan Sauteraud, and Raphael Gottardo
Part II: Chemoselective Strategies to Peptide Immobilization
11. Chemoselective Strategies to Peptide and Protein Bioprobes Immobilization on Microarray Surfaces
Alessandro Gori and Renato Longhi
12. Manufacturing of Peptide Microarrays Based on Catalyst-Free Click Chemistry
Denis Prim and Marc E. Pfeifer
13. Clickable Polymeric Coating for Oriented Peptide Immobilization
Laura Sola, Alessandro Gori, Marina Cretich, Chiara Finetti, Caterina Zilio, and Marcella Chiari
14. Oriented Peptide Immobilization on Microspheres
Lisa C. Shriver-Lake, George P. Anderson, and Chris R. Taitt
15. A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis
Kenji Usui, Kin-ya Tomizaki, and Hisakazu Mihara
Part III: Peptide Microarrays for Medical Applications
16. Peptide Microarrays for
Medical Applications in Autoimmunity, Infection, and Cancer Carsten Grötzinger
17. Synthetic Peptide-Based ELISA and ELISpot Assay for Identifying Autoantibody Epitopes
Judit Pozsgay, Eszter Szarka, Krisztina Huber, Fruzsina Babos, Anna Magyar, Ferenc Hudecz, and Gabriella Sarmay
18. IgE and IgG4 Epitope Mapping of Food Allergens with a Peptide Microarray Immunoassay
Javier Martínez-Botas and Belén de la Hoz
19. IgE Epitope Mapping Using Peptide Microarray Immunoassay
Gustavo Gimenez, Sara Benedé, and Jing Lin
20. Spot Synthesis: An Optimized Microarray to Detect IgE Epitopes
Salvatore G. De-Simone and Paloma Napoleão-Pêgo
21. Mapping of Epitopes Occurring in Bovine αs1-Casein Variants by Peptide Microarray Immunoassay
Maria Lisson and Georg Erhardt
