Part I: Sampling, Chromatographic Purification, and Chemical Analysis of Pheromone Molecules

1. Female Moth Pheromones

Tetsu Ando

2. Pheromones in the Fruit Fly

Jacqueline Chin and Joanne Y. Yew

3. Analysis of Volatile Mouse Pheromones by Gas Chromatography Mass Spectrometry

Milos V. Novotny and Helena A. Soini

4. Murine Non-Volatile Pheromones: Isolation of Exocrine-Gland Secreting Peptide 1

Hiroko Kimoto and Kazushige Touhara

5. Chemical Analysis of Aquatic Pheromones in Fish

Michael Stewart, Cindy F. Baker, and Peter W. Sorensen

6. Analysis of Ascarosides from Caenorhabditis elegans Using Mass Spectrometry and NMR Spectroscopy

Xinxing Zhang, Jaime H. Noguez, Yue Zhou, and Rebecca A. Butcher

Part II: Approaches to Characterize Gene and Function of Pheromone Receptors

7. Identification of Chemosensory Receptor Genes from Vertebrate Genomes

Yoshihito Niimura

8. Functional Assays for Insect Olfactory Receptors in Xenopus Oocytes

Tatsuro Nakagawa and Kazushige Touhara

9. A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors

Sandeepa Dey, Senmiao Zhan, and Hiroaki Matsunami

10. Genetic Manipulation to Analyze Pheromone Responses: Knockouts of Multiple Receptor Genes

Tomohiro Ishii

Part III: Methods in Measuring Neural Responses to Pheromones

11. Electro-Antennogram and Single Sensillum Recording in Insect Antennae

Shannon B. Olsson and Bill S. Hansson

12. Calcium Imaging of Pheromone Responses in the Insect Antennal Lobe

Susy M. Kim and Jing W. Wang

13. Live Cell Calcium Imaging of Dissociated Vomeronasal Neurons

Angeldeep Kaur, Sandeepa Dey, and Lisa Stowers

14. Whole Mount Imaging of Responses in Mouse Vomeronasal Neurons

Pei Sabrina Xu and Timothy E. Holy

15. Calcium Imaging of Vomeronasal Organ Response Using Slice Preparations from Transgenic Mice Expressing G-CaMP2

C. Ron Yu

16. The Electrovomeronasogram: Field Potential Recordings in the Mouse Vomeronasal Organ

Trese Leinders-Zufall and Frank Zufall

17. Electrical Recordings from the Accessory Olfactory Bulb in VNO-AOB Ex Vivo Preparations

Julian P. Meeks and Timothy E. Holy

18. Pheromone-Induced Expression of Immediate Early Genes in the Mouse Vomeronasal Sensory System

Sachiko Haga-Yamanaka and Kazushige Touhara

Part IV: Protocols in Analyzing Behavioral and Endocrine Changes Elicited by Pheromones

19. Insect Pheromone Behavior: Fruit Fly

Daisuke Yamamoto, Soh Kohatsu, and Masayuki Koganezawa

20. Quantitative Assessment of Pheromone-Induced Dauer Formation in Caenorhabditis elegans

Scott J. Neal, Kyuhyung Kim, and Piali Sengupta

21. Acute Behavioral Responses to Pheromones in C. elegans (Adult Behaviors: Attraction, Repulsion)

Heeun Jang and Cornelia I. Bargmann

22. Behavioral Analysis of Pheromones in Fish

Peter W. Sorensen

23. Analysis of Male Aggressive and Sexual Behavior in Mice

Takefumi Kikusui

24. Assessment of Urinary Pheromone Discrimination, Partner Preference, and Mating Behaviors in Female Mice

Olivier Brock, Julie Bakker, and Michael J. Baum

25. Assessing Postpartum Maternal Care, Alloparental Behavior, and Infanticide in Mice: With Notes on Chemosensory Influences

Kumi O.