1. The Phytoplasmas - An Introduction
Matt Dickinson, Melanie Tuffen, and Jennifer Hodgetts
2. Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus
Jennifer Hodgetts, David Crossley, and Matt Dickinson
3. Micropropagation and Maintenance of Phytoplasmas in Tissue Culture
Assunta Bertaccini, Samanta Paltrinieri, Marta Martini, Mara Tedeschi, and Nicoletta Contaldo
4. Dodder Transmission of Phytoplasmas
Jaroslava Přibylová and Josef Spak
5. Insect Maintenance and Transmission
Heather Kingdom
6. Capturing Insect Vectors of Phytoplasmas
Phyllis Weintraub and Jürgen Gross
7. Insect Vector Transmission Assays
Domenico Bosco and Rosemarie Tedeschi
8. Molecular Identification of Phytoplasma Vector Species
Sabrina Bertin and Domenico Bosco
9. Dienes' Staining and Light Microscopy for Phytoplasma Visualization
Rita Musetti
10. DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas
Nancy S. Andrade and Nolberto S. Arismendi
11. Visualisation of Phytoplasmas using Electron Microscopy
B. Jean Devonshire
12. Automated DNA Extraction for Large Numbers of Plant Samples
Natasa Mehle, Petra Nikolic, Matevz Rupar, Jana Boben, Maja Ravnikar, and Marina Dermastia
13. DNA Extraction from Arborescent Monocots and How To Deal With Other Challenging Hosts
Nigel A. Harrison, Robert E. Davis, and Ericka E. Helmick
14. Nested PCR and RFLP Analysis Based on the 16S rRNA Gene
Bojan Duduk, Samantha Paltrinieri, Ing Ming Lee, and Assunta Bertaccini
15. PCR and RFLP Analyses Based on the Ribosomal Protein Operon
Marta Martini and Ing-Ming Lee
16. Tuf and secY PCR Amplification and Genotyping of Phytoplasmas
Xavier Foissac, Jean-Luc Danet, Sylvie Malembic-Maher, Pascal Salar, Dana Safářová, Pavla Válová, and Milan Navrátil
17. PCR Analysis of Phytoplasmas Based on the secA Gene
Matt Dickinson and Jennifer Hodgetts
18. Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains
Martina Seruga Music and Dijana Skoric
19. Microarrays for Universal Detection and Identification of Phytoplasmas
Mogens Nicolaisen, Henriette Nyskjold, and Assunta Bertaccine
20. T-RFLP for Detection and Identification of Phytoplasmas in Plants
Jennifer Hodgetts and Matt Dickinson
21. Real-Time PCR for Universal Phytoplasma Detection and Quantification
Nynne Meyn Christensen, Henriette Nyskjold, and Mogens Nicolaisen
22. A Real-Time PCR Detection System for the Bois Noir and Flavescence Dorée Phytoplasmas and Quantification of the Target DNA
Natasa Mehle, Nina Prezelj, Matjaz Hren, Jana Boben, Kristina Gruden, Maja Ravnikar, and Marina Dermastia
23. Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation Group
Natasa Mehle, Petra Nikolic, Kristina Gruden, Maja Ravnikar, and Marina Dermastia
24. Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions
Paola Margaria and Sabrina Palmano
25. In-Field Diagnostics Using Loop-Mediated Isothermal Amplification (LAMP)
Jenny Tomlinson
26. DNA Bar-Coding
