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Name: Phytoplasmas
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ISBN: 9781493988365

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Phytoplasmas

(Hardback) | Released: 26 Oct 2018

By: Rita Musetti (Edited) , Laura Pagliari (Edited) | Publisher: Humana Press | Publisher Imprint: Humana Press

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About the Book

1. Phytoplasmas: An Introduction

L. Pagliari and R. Musetti

Part II: Preparing Plant Material

2. Micro-tom Tomato Grafting for Stolbur-phytoplasma Transmission: Different Grafting Techniques

Sara Buoso and Alberto Loschi

3. Phytoplasma Transmission: Insect Rearing and Infection Protocols

L. Pagliari, J. Chuche, D. Bosco^, and D. Thiéry

4. Sampling Methods for Leafhopper, Planthopper, and Psyllid Vectors

Kerstin Krüger and Nicola Fiore

5. Symptoms of Phytoplasma Diseases

Paolo Ermacora and Ruggero Osler

Part II: Molecular Analyses 6. Comparison of Different Procedures for DNA Extraction for Routine Diagnosis of Phytoplasmas Carmine Marcone

7. Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene

Assunta Bertaccini, Samanta Paltrinieri and Nicoletta Contaldo

8. PCR-based Sequence Analysis on Multiple Genes other than 16S rRNA Gene for Differentiation of Phytoplasmas

Marta Martini, Kristi D. Bottner-Parker, and Ing-Ming Lee

9. Real-time PCR Protocol for Phytoplasma Detection and Quantification

Yusuf Abou-Jawdah, Vicken Aknadibossian, Maan Jawhari, Patil Tawidian, and Peter Abrahamian

10. Duplex TaqMan Real-Time PCR for Rapid Quantitative Analysis of a Phytoplasma in its Host Plant without External Standard Curves

Sanja Baric

11. A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas

Shigeyuki Kakizawa

12. One-step Multiplex Quantitative RT-PCR for the Simultaneous Detection of Viroids and Phytoplasmas

Ioanna Malandraki, Christina Varveri and Nikon Vassilakos

13. A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification

Claudio Ratti, Stefano Minguzzi, and Massimo Turina

14. Quantitative Analysis with Droplet Digital PCR

Natasa Mehle and Tanja Dreo

15. Rapid Sample Preparation and LAMP for Phytoplasma Detection

Jennifer Hodgetts

16. Assembly of Phytoplasma Genome Drafts from Illumina Reads using Phytoassembly

Cesare Polano and Giuseppe Firrao

17. Protocol for the Definition of a Multi-Spectral Sensor for Specific Foliar Disease Detection: Case of 'Flavescence Dorée'

H. Al-Saddik, A. Laybros, JC. Simon, and F. Cointault

18. Transcriptomic Analyses of Phytoplasmas

Davide Pacifico, Simona Abbà, and Sabrina Palmano

Part III: Site-specific Analyses

19. Transcriptomic Analyses of Phytoplasmas

Davide Pacifico, Simona Abbà, and Sabrina Palmano

20. Laser Microdissection of Phytoplasma-infected Grapevine Leaf Phloem Tissue for Gene Expression Study

Simonetta Santi

21. Collection of Phloem Sap in Phytoplasma-infected Plants

Matthias R. Zimmermann, Torsten Knauer and, Alexandra C.U. Furch

22. DAPI and Confocal Laser-Scanning Microscopy for in vivo Imaging of Phytoplasmas

Rita Musetti and Stefanie Vera Buxa

23. Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas

Luciana Galetto, Marta Vallino, Mahnaz Rashidi, and Cristina Marzachì

Part IV: Plant-pathogen Interaction

24. Characterization of Phytoplasmal Effector Protein Interaction with Proteinaceous Plant Host Targets using Bimolecular Fluorescence Complementation (BiFC)

Katrin Janik, Hagen Stellmach, Cecilia Mittelberger, and Bettina Hause

25. Col

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Product Details

ISBN-13: 9781493988365
Publisher: Humana Press
Publisher Imprint: Humana Press
Height: 254 mm
No of Pages: 362
Series Title: Methods in Molecular Biology
Sub Title: Methods and Protocols
Width: 178 mm

ISBN-10: 1493988360
Publisher Date: 26 Oct 2018
Binding: Hardback
Language: English
Returnable: Y
Spine Width: 22 mm
Weight: 915 gr

Related Categories

Science & Mathematics > Biology, life sciences > Botany & plant sciences > Plant physiology

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