1. Essential Methods of Plant Sample Preparation for Light Microscopy
Ales Soukup and Edita Tylová
2. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy
Ales Soukup
3. Resin Embedding, Sectioning and Immunocytochemical Analyses of Plant Cell Walls in Hard Tissues
Kieran J. D. Lee and J. Paul Knox
4. Automated Microscopy in Forward Genetic Screening of Arabidopsis
Tereza Dobisová and Jan Hejátko
5. Image Analysis: Basic Procedures for Description of Plant Structures on Anatomical Sections
Jana Albrechtová, Zuzana Kubínová, Ales Soukup, and Jiří Janáček
6. Identifying Subcellular Protein Localization with Fluorescent Protein Fusions After Transient Expression in Onion Epidermal Cells
Andreas Nebenführ
7. Visualizing and Quantifying the in vivo Structure and Dynamics of the Arabidopsis Cortical Cytoskeleton using CLSM and VAEM
Amparo Rosero, Viktor Zárský, and Fatima Cvrčková
8. Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces
Dorota Kwiatkowska and Agata Burian
9. Time-lapse Imaging of Developing Meristems using Confocal Laser Scanning Microscope
Olivier Hamant, Pradeep Das, and Agata Burian
10. Quantifying Cell Shape and Gene Expression in the Shoot Apical Meristem using MorphoGraphX
Pierre Barbier de Reuille, Sarah Robinson, and Richard S. Smith
11. Mechanical Measurements on Living Plant Cells by Micro-indentation with Cellular Force Microscopy
Anne-Lise Routier-Kierzkowska and Richard S. Smith
12. High-pressure Freezing and Low-temperature Processing of Plant Tissue Samples for Electron Microscopy
Ichirou Karahara and Byung-Ho Kang
13. Reconstructing Plant Cells in 3D by Serial Section Electron Tomography
Kiminori Toyooka and Byung-Ho Kang
14. Imaging Plant Nuclei and Membrane-associated Cytoskeleton by Field Emission Scanning Electron Microscopy
Jindřiska Fiserová and Martin W. Goldberg
15. Immuno-gold Labeling of Resin-embedded Electron Microscopical Sections
Ilse Foissner and Margit Hoeftberger
16. Live Cell Imaging of Arabidopsis Root Hairs
Tijs Ketelaar
17. Morphological Analysis of Cell Growth Mutants in Physcomitrella
Luis Vidali and Jeffrey P. Bibeau
18. Plant Cell Lines in Cell Morphogenesis Research
Daniela Seifertová, Petr Klíma, Markéta Pařezová, Jan Petrásek, Eva Zazímalová, and Zdeněk Opatrný
19. Antisense Oligodeoxynucleotide-mediated Gene Knock-down in Pollen Tubes
Radek Bezvoda, Roman Pleskot, Viktor Zárský, and Martin Potocký
20. Lab-on-a-Chip for Studying Growing Pollen Tubes
Carlos G. Agudelo, Muthukumaran Packirisamy, and Anja Geitmann
21. Laser Microdissection of Plant Cells
Yvonne Ludwig and Frank Hochholdinger
22. Optical Trapping in Plant Cells
Tijs Ketelaar, Norbert de Ruijter, and Stefan Niehren
23. Heterologous Expression in Budding Yeast as a Tool for Studying the Plant Cell Morphogenesis Machinery
Fatima Cvrčková and Michal Hála
