Section I: Automation and Imaging
1. Fully-automated Compound Screening in Arabidopsis thaliana Seedlings
Dominique Audenaert, Long Nguyen, Bert De Rybel, and Tom Beeckman
2. Time-profiling Fluorescent Reporters in the Arabidopsis Root
Antoine P. Larrieu, Andrew P. French, Tony P. Pridmore, Malcolm J. Bennett and Darren M. Wells
3. Screening for Bioactive Small Molecules by in vivo Monitoring of Luciferase-based Reporter Gene Expression in Arabidopsis thaliana
Christian Meesters and Erich Kombrink
Section II: Chemical Screening
4. Application of Yeast-two Hybrid Assay to Chemical Genomic Screens: A High-throughput System to Identify Novel Molecules Modulating Plant Hormone Receptor Complexes
Andrea Chini
5. High-throughput Screening of Small Molecule Libraries for Inducers of Plant Defense Responses
Colleen Knoth and Thomas Eulgem
6. Using a Reverse Genetics Approach to Investigate Small Molecule Activity
Siamsa M. Doyle and Stéphanie Robert
7. Investigating the Phytohormone Ethylene Response Pathway by Chemical Genetics
Lee-Chung, Lin, Chiao-Mei Chueh, and Long-Chi Wang
8. Screening for Inhibitors of Chloroplast Galactolipid Synthesis Acting in membrano and in Planta
Laurence Boudière and Eric Maréchal
9. Forward Chemical Screening of Small RNA Pathways
Yifan Lii and Hailing Jin
10. Identification and Use of Fluorescent Dyes for Plant Cell Wall Imaging Using High-Throughput Screening
Charles T. Anderson and Andrew Carroll
11. High-throughput Identification of Chemical Endomembrane Cycling Disruptors Utilizing Tobacco Pollen
Michelle Q. Brown, Nolan Ung, Natasha V. Raikhel, and Glenn R. Hicks
12. Plant Chemical Genomics: Gravity Sensing and Response
Marci Surpin
13. Screening Chemical Libraries for Compounds that Affect Protein Sorting to the Yeast VacuoleJan Zouhar
Section III: Cheminformatics
14. The use of Multi-drug Approach to Uncover New Players of the Endomembrane System Trafficking Machinery
Urbina D, Pérez-Henríquez P, and Norambuena L
15. Cheminformatic Analysis of High-Throughput Compound ScreensTyler W. H. Backman and Thomas Girke
16. Endomembrane Dissection Using Chemically-Induced Bioactive Clusters
Natasha Worden, Thomas Girke, and Georgia Drakakaki
17. Statistical Molecular Design: A Tool to Follow Up Hits From Small Molecule ScreeningAnders E. G. Lindgren, Andreas Larsson, Anna Linusson, and Mikael Elofsson
Section IV: Target Identification
18. Early Stage Hit Triage for Plant Chemical Genetic Screens and Target Site Identification
Terence A. Walsh
19. Screening for Gene Function using the FOX (Full-length cDNA OvereXpressor gene) Hunting System
Mieko Higuchi, Minami Matsui
Section V: Hormone Transport and Metabolite Profiling
20. Quantification of Stable Isotope Label in Metabolites via Mass Spectrometry
Jan Huege, Jan Goetze, Frederik Dethloff, Bjoern Junker, and Joachim Kopka
21. 1H NMR Based Metabolomics Methods for Chemical Genomics Experiments
Daniel J. Orr, Gregory A. Barding, Jr., Christiana E. Merrywell, Glenn R. Hicks, Natasha V. Raikhel, and Cynthia K. Larive
22. Determination of Auxin Transport Parameters on the Cellular Level
Jan Petrásek, Martina Laňková, and Eva Zazímalová
23. Analyzing the in vivo Status of Exogenously Applied Auxins: A HPLC-based Method to Characterize the Intracellularly Localized Auxin Transporters
Sibu Simon, Petr Skůpa, Petre I. Dobrev, Jan Petrásek, Eva Zazímalová, and Jiří Friml
