Plant Gametogenesis

Part I: Omics Approaches to Study Male and Female Germlines in Plants

1. Measurement of flower metabolite concentrations using Gas-Chromatography Mass Spectrometry and High-Performance Liquid Chromatography Mass Spectrometry

Monica Borghi, Leonardo Perez de Souza, and Alisdair R. Fernie

2. Proteomic-, phosphoproteomic- and Acetylomic-based mass spectrometry to identify tissue-specific protein complexes and phosphorylation in plant gametogenesis

Lu Sun, Aowei Mo, and Pingli Lu

3. Identifying small RNAs and analyzing their association with gene expression using isolated Arabidopsis male meiocytes

Jiyue Huang, Chenjiang You, Cong Wang, Yingxiang Wang, and Gregory P. Copenhaver

4. CTAB DNA extraction and genotyping-by-sequencing to map meiotic crossovers in plants

Pallas C. Kuo, Ian R. Henderson, and Christophe Lambing

Wenjing Wang, Longjian Niu, Chunhui Hou

Part II: Microscopy Approaches To Study Gametogenesis In Plants

6. Cytogenetic techniques for analysing meiosis in hexaploidy bread wheat

Kim Osman, F. Chris H. Franklin, and Eugenio Sanchez-Moran

7. Super-resolution chromatin visualisation by combined fluorescence in situ hybridisation and Structured Illumination Microscopy in Solanum lycopersicum

Pallas C. Kuo, Alice Darbyshire, and Christophe Lambing

8. Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy

Sona Valuchova, Pavlina Mikulkova, Jana Pecinkova, and Karel Riha

9. Bimolecular Fluorescence Complementation to Test for Protein-Protein Interactions and To Uncover Regulatory Mechanisms During Gametogenesis

Ramakrishna Yadala, Maryia Ratnikava, and Inna Lermontova

10. Quantifying meiotic crossover recombination in Arabidopsis lines expressing fluorescent reporters in seeds using SeedScoring pipeline for CellProfiler

Nadia Kbiri, Julia Dluzewska, Ian R. Henderson, and Piotr A. Ziolkowski

Part III: Plant Manipulation and Genome Editing

11. Generation of mutants by combined treatment of physical and chemical mutagens in rice

Yuanyuan Tan andShan Li

12. Generation of mutant plants by Gamma Ray exposure and development of low-cost TILLING population in chickpea (Cicer arietinum L.)

WahibaAmri-Tiliouine, Joanna Jankowicz-Cieslak, Bradley J. Till, and Meriem Laouar

13. Efficient generation of CRISPR/Cas9-based mutants supported by fluorescent seed selection in different Arabidopsis accessions

Tomasz Bieluszewski, Maja Szymanska-Lejman, Wojciech Dziegielewski, Longfei Zhu, and Piotr A. Ziolkowski

14. Generation of deletion lines in Allohexaploid Bread Wheat

Heïdi Serra, Radim Svačina, Jan Bartos, and Pierre Sourdille

15. Post-transcriptional Gene Silencing Using Virus-Induced Gene Silencing to Study Plant Gametogenesis in Tomato

Zhengming Wang, Xiao Yu Wang, Claudia Martinho, and David C. Baulcombe

16. Protocols for in vivo doubled haploid (DH) technology in maize breeding: From haploid inducer Development to Haploid Genome Doubling

Siddique I. Aboobucker, Talukder Z. Jubery, Ursula K. Frei, Yu-Ru Chen, Tyler Foster, Baskar Ganapathysubramanian, and Thomas Lübberstedt

17. Estimation of heterografting associated DNA methylation changes in tree crops by MSAP analysis

Thomas Kadampanattu Uthup