1. Plant Isoprenoids: A General Overview
Manuel Rodríguez-Concepción
I. Measurement of Core Enzyme Activities
2. Measuring the Activity of 1-Deoxy-D-Xylulose 5-Phosphate Synthase, the First Enzyme in the MEP Pathway, in Plant Extracts
Louwrance P. Wright and Michael A. Phillips
3. Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants
Narciso Campos, Montserrat Arró, Albert Ferrer, and Albert Boronat
4. Farnesyl Diphosphate Synthase Assay
Montserrat Arró, David Manzano, and Albert Ferrer
II. Targeted Analysis of Isoprenoid Metabolites
5. Metabolite Profiling of Plastidial Deoxyxylulose-5-Phosphate Pathway Intermediates by Liquid Chromatography and Mass Spectrometry
Edward E.K. Baidoo, Yanmei Xiao, Katayoon Dehesh, and Jay D. Keasling
6. Analysis of Carotenoids and Tocopherols in Plant Matrices and Assessment of Their In Vitro Antioxidant Capacity
Antonio J. Meléndez-Martínez, Carla M. Stinco, Paula Mapelli Brahm, and Isabel M. Vicario
7. Simultaneous Analyses of Oxidized and Reduced Forms of Photosynthetic Quinones by High-Performance Liquid Chromatography
Masaru Shibata and Hiroshi Shimada
8. Determination of Sterol Lipids in Plant Tissues by Gas Chromatography and Q-TOF Mass Spectrometry
Vera Wewer and Peter Dörmann
9. Analysis of Plant Polyisoprenoids
Katarzyna Gawarecka and Ewa Swiezewska
10. Analysis of Diterpenes and Triterpenes from Plant Foliage and Roots
Qiang Wang, Reza Sohrabi, and Dorothea Tholl
11. Gas Chromatography Mass-Spectrometry Method for Determination of Biogenic Volatile Organic Compounds Emitted by Plants
Astrid Kännaste, Lucian Copolovici, and Ülo Niinemets
12. Analysis of Steroidal Alkaloids and Saponins in Solanaceae Plant Extracts using UPLC-qTOF Mass Spectrometry
Uwe Heinig and Asaph Aharoni
III. Isoprenoid Profiling in Specialized Organs
13. Isoprenoid and Metabolite Profiling of Plant Trichomes
Gerd Ulrich Balcke, Stefan Bennewitz, Sebastian Zabel, and Alain Tissier
14. Sample Preparation for Single Cell Transcriptomics - Essential Oil Glands in Citrus Fruit Peel as an Example
Siau Sie Voo and Bernd Markus Lange
15. Prenylquinone Profiling in Whole Leaves and Chloroplast Subfractions
Felix Kessler and Gaetan Glauser
16. Confocal Laser Scanning Microscopy Detection of Chlorophylls and Carotenoids in Chloroplasts and Chromoplasts of Tomato Fruit
Lucio D'Andrea, Montse Amenós, and Manuel Rodríguez-Concepción
IV. Genetic, Pharmacological, and Bioinformatic Tools
17. Heterologous Expression of Triterpene Biosynthetic Genes in Yeast and Subsequent Metabolite Identification Through GC-MS
Ery O. Fukushima, Hikaru Seki, and Toshiya Muranaka
18. High Throughput Testing of Terpenoid Biosynthesis Candidate Genes Using Transient Expression in Nicotiana benthamiana
Søren Spanner Bach, Jean-Étienne Bassard, Johan Andersen-Ranberg, Morten Emil Møldrup, Henrik Toft Simonsen, and Björn Hamberger
19. Heterologous Stable Expression of Terpenoid Biosynthetic Genes Using the Moss Physcomitrella patens
Søren Spanner Bach, Brian Christopher King, Xin Zhan, Henrik Toft Simonsen, and Björn Hamberger
20. Quantification of Plant Resistance to Isoprenoid Biosynthesis Inhibitors
Catalina Perelló, Manuel Rodríguez-Concepción, and Pablo Pulido
21. A Flexible Protocol for Targeted Gene Co-Expression Network Analysis
Diana Coman, Philipp Rütimann, and Wilhelm Gruissem
