Part I: Lipid Analyses
1. Analyzing Plant Signaling Phospholipids through 32Pi-Labeling and TLC
Teun Munnik & Xavier Zarza
2. Analysis of D3-,4-,5-phosphorylated Phosphoinositides using HPLC
Teun Munnik
3. Mass Measurement of Polyphosphoinositides by Thin Layer- and Gas Chromatography
Mareike Heilmann and Ingo Heilmann
4. Measurement of Inositol (1,4,5) Trisphosphate in Plant Tissues by a Competitive Receptor Binding Assay
Ingo Heilmann and Imara Y. Perera
5. Quantification of Diacylglycerol by Mass Spectrometry
Katharina vom Dorp, Isabel Dombrink and Peter Dörmann
6. Distinguishing Phosphatidic Acid Pools from de novo Synthesis, PLD and DGK
Steven A. Arisz & Teun Munnik
7. Use of Phospholipase A2 for the Production of Lysophospholipids
Steven A. Arisz & Teun Munnik
8. Analysis and Quantification of Plant Membrane Lipids by Thin-Layer Chromatography and Gas Chromatography
Vera Wewer, Peter Dörmann & Georg Hölzl
9. Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass Spectrometry
Sunitha Shiva, Hieu Sy Vu, Mary R. Roth, Zhenguo Zhou, Shantan Reddy Marepally, Daya Sagar Nune, Gerald H. Lushington, Mahesh Visvanathan, and Ruth Welti
10. Detection and Quantification of Plant Sphingolipids by LC-MS
Jonathan E. Markham
11. Analysis of Defense Signals in Arabidopsis thaliana Leaves by Ultra Performance Liquid Chromatography/ Tandem Mass Spectrometry: Jasmonates, Salicylic Acid, Abscisic Acid
Nadja Stingl, Markus Krischke, Agnes Fekete and Martin J. Mueller
12. Analysis of Fatty Acid Amide Hydrolase (FAAH) Activity in Plants
Sang-Chul Kim, Lionel Faure, and Kent D. Chapman
13. Ionization Behavior of Polyphosphoinositides Determined via the Preparation of pH Titration Curves using Solid State 31P NMR
Zachary T. Graber, and Edgar E. Kooijman
Part II: Enzyme Activities
14. Phosphatidylinositol Synthase Activity from Plant Membrane Fractions and from E. coli-Expressed Recombinant Peptides
Sylvie Collin and Françoise Cochet
15. Phosphatidylinositol 4-kinase and Phosphatidylinositol 4-phosphate 5-kinase Assays
Yang Ju Im, Irena Brglez, Catherine Dieck, Imara Perera and Wendy F. Boss
16. Assaying Inositol and Phosphoinositide Phosphatase Enzymes
Janet L. Donahue, Mustafa Ercetin and Glenda E. Gillaspy
17. Determination of Phospholipase C Activity In Vitro
S.M. Teresa Hernández-Sotomayor and J. Armando Muñoz-Sanchez
18. Assaying Non-Specific Phospholipase C (NPC) Activity
Přemysl Pejchar, Günther F. E. Scherer and Jan Martinec
19. Assaying Different Types of Plant Phospholipase D Activities In Vitro
Kirk L. Pappan and Xuemin Wang
20. Measuring PLD Activity In Vivo
Teun Munnik & Ana M. Laxalt
21. Assaying Plant Phosphatidic Acid Phosphatase Activity
Yuki Nakamura
22. Assay of Phospholipase A Activity
Michael Heinze and Werner Roos
Jonathan E. Markham
Part III: Protein-Lipid Intractions
23. Lipid-Binding Analysis using a Fat Blot Assay
Teun Munnik and Magdalena Wierzchowiecka
24. Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein Interactions
Magdalena M. Julkowska, Johanna M. Rankenberg and Christa Testerink
25. Lipid affinity beads - From Identifying New Lipid Binding Proteins to Assessing their Binding Properties
Fionn McLoughlin & Christa Testerink
Part IV: Imaging
26. Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living Plants
Joop E.M. Vermeer and Teun Munnik
27. Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and