Section I: CYTOLOGICAL TECHNIQUES FOR LIGHT MICROSCOPY
1. Spreading and Fluorescence in situ Hybridization (FISH) of Male and Female Meiocyte Chromosomes from Arabidopsis thaliana for Cytogenenetical Analysis
Susan Armstrong
2. Analysis of Plant Meiotic Chromosomes by Chromosome Painting
Martin A. Lysak and Terezie Mandáková
3. Using Sequential Fluorescence and Genomic in situ Hybridization (FISH and GISH) to Distinguish the A and C Genomes in Brassica napus
Elaine C. Howell and Susan Armstrong
4. Labeling Meiotic Chromosomes in Maize with Fluorescence in situ Hybridization
Zhi Gao, Fangpu Han, Tatiana V. Danilova, Jonathan C. Lamb, Patrice S. Albert, and James A.
Birchler
5. Examining Female Meiocytes of Maize by Confocal Microscopy
Philippa Barrell and Ueli Grossniklaus
6. Three-dimensional Acrylamide Fluorescence in situ Hybridization (FISH) for Plant Cells
Elizabeth S. Howe, Shaun P. Murphy, and Hank W. Bass
7. Analyzing Maize Meiotic Chromosomes with Super-resolution Structured Illumination Microscopy
Chung-Ju Rachel Wang
8. Live Imaging of Chromosome Dynamics
Moira J. Sheehan, R. Kelly Dawe, and Wojciech P. Pawlowski
9. Immunolocalization of Meiotic Proteins in Brassicacae - Method 1
Liudmila A. Chelysheva, Laurie Grandont, Mathilde Grelon
10. Immunolocalization of Meiotic Proteins in Arabidopsis thaliana - Method 2
Susan Armstrong and Kim Osman
11. Immunolocalization Protocols for Visualizing Meiotic Proteins in Arabidopsis thaliana - Method 3
Xiaohui Yang, Li Yuan and Christopher A, Makaroff
12. A Time Course for the Analysis of Meiotic Progression in Arabidopsis thaliana
Susan Armstrong
13. Analyzing Meiotic Chromosomes in Rice
Zhukuan Cheng
14. Analysing Meiosis in Barley
James D. Higgins
Section II: CYTOLOGICAL TECHNIQUES FOR ELECTRON MICROSCOPY
15. Preparing SC Spreads with RNs for EM Analysis
Lorinda K. Anderson and Stephen M. Stack
16. Analysis of the Synaptonemal Complex in Brassica using TEM
Susan Armstrong
17. Preparing Thin Sections of Meiotic Nuclei for Transmission Electron Microscopy
Ljudmilla Timofejeva
Section III: GENETICS AND MOLECULAR BIOLOGY TECHNIQUES
18. Characterization of Meiotic Non-crossover Molecules from Arabidopsis thaliana Pollen Hossein Khademian, Laurène Giraut, Jan Drouaud, and Christine Mézard
19. Chromatin Immunoprecipitation for Studying Chromosomal Localization of Meiotic Proteins in Maize
Yan He, Gaganpreet Sidhu, and Wojciech P. Pawlowski
20. Analyzing the Meiotic Transcriptome using Isolated Meiocytes of Arabidopsis thaliana
Changbin Chen and Ernest F. Retzel
21. Analysis of Meiotic Protein Complexes from Arabidopsis and Brassica using Affinity-based Proteomics
Kim Osman, Elisabeth Roitinger, Jianhua Yang, Susan Armstrong, Karl Mechtler, and F. Chris H. Franklin
22. Identifying Meiotic Mutants in Arabidopsis thaliana
Wayne Crismani and Raphaël Mercier
About the Author:
Meiosis is one of the most critical processes in eukaryotes, required for continuation of species and generation of new variation. In plants, meiotic recombination is by far the most important source of genetic variation. In Plant Meiosis: Methods and Protocols, expert researchers in the field detail methods for molecular cytogenetics and chromosome analysis in plants. These methods and protocols allows for understand the organization and behavior of the genetic material in a wide range of both model and crop species. extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Meiosis: Methods and Protocols provides and extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.
