Preface...
Table of Contents...
Contributing Authors...
1. An Overview of Protein Secretion in Yeast and Animal Cells
Yusong Guo, Feng Yang, and Xiao Tang
2. An Overview of Protein Secretion in Plant Cells
Kin Pan Chung and Yonglun Zeng
3. Bioinformatics Analysis of Protein Secretion in Plants
Liyuan Chen
4. Proteomics Analysis of Secreted Proteins from Cell Microenvironment
Subash Adhikari, Lan Chen, Peiwu Huang, and Ruijun Tian
5. Using Homology Modelling to Understand the Structural Basis of Specific Interaction of a Plant-Specific AtSar1a/AtSec23a Pair Involved in Protein ER Export
Yap-Shing Nim, Shuangli Sun, and Kam-Bo Wong
6. Analysis of Golgi-Mediated Protein Traffic in Plant Cells
Wenjin Shen, Zhidan Xiao, Jinbo Shen, and Caiji Gao
7. Analysis of Membrane Protein Topology in the Plant Secretory Pathway
Jinya Guo, Yansong Miao, and Yi Cali
8. Semi-Automatic Segmentation of Plant Golgi Stacks in Electron Tomograms Using 3dmod
Keith Ka Ki Mai and Byung-Ho Kang
9. 3D Printing of Plant Golgi Stacks from their Electron Tomographic Models
Keith Ka Ki Mai, Madison J. Kang, and Byung-Ho Kang
10. Transient Expression of Chimeric Fluorescent Reporter Proteins in Pollen Tubes to Study Protein Polar Secretion and Dynamics
Guitao Zhong, Ronghe Liu, Menglong Zhuang, and Hao Wang
11. Analysis of Actin-Based Intracellular Trafficking in Pollen Tubes
Yuxiang Jiang, Meng Zhang, and Shanjin Huang
12. Analysis of Phragmoplast Kinetics during Plant Cytokinesis
Pantelis Livanos, Mayank Chugh, and Sabine Müller
13. Immunofluorescence Analysis of Membrane-Associated Proteins for Clathrin-Mediated Endocytosis in Plant Root Cells
Chao Wang, Xu Yan, Tingting Meng, Tianwei Hu, and Jianwei Pan
14. In Vivo Interaction Studies by Measuring Förster Resonance Energy Transfer through Fluorescence Lifetime Imaging Microscopy (FRET/FLIM)
Florian Fäßler and Peter Pimpl
15. Analysis of Nanobody-Epitope Interactions in Living Cells via Quantitative Protein Transport Assays
Simone Früholz and Peter Pimpl
16. A Secretion System for Identification of Cargo Proteins of Vacuolar Sorting Receptors
Jinbo Shen
17. Identifying Novel Regulators of Vacuolar Trafficking by Combining Fluorescence Imaging-Based Forward Genetic Screening and In Vitro Pollen Germination
Qiang-Nan Feng and Yan Zhang
18. Measuring Plant Protein Secretion
Emily R. Larson
19. Transient Secretory Enzyme Expression in Leaf Protoplasts to Characterize SNARE Functional Classes in Conventional and Unconventional Secretion
Di Sansebastiano Gian Pietro and Barozzi Fabrizio
20. The Organelle pH Estimate and Measurement in Plant Secretory Pathway
Jinbo Shen
21. Analysis of Exocyst-Positive Organelle (EXPO)-Mediated Unconventional Protein Secretion in Plant Cells
Yu Ding and Juan Wang
22. Isolation of the Plant Exocyst Complex
King Pong Leung and Wilson Chun Yu Lau
23. Using
