1. Experimental Analysis of Receptor Kinase Phosphorylation
Srijeet K. Mitra, Michael B. Goshe, and Steven D. Clouse
2. Quantitative Measurement of Phosphopeptides and Proteins via Stable Isotope Labeling in Arabidopsis (SILIA) and Functional Phosphoproteomic Strategies
Ning Li
3. Identification of O-GlcNAc Modified Proteins from Arabidopsis
Shou-Ling Xu, Robert J. Chalkley, Zhi-Yong Wang, and Alma L. Burlingame
4. Quantitative Analysis of Protein Phosphorylation Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE)
Zhiping Deng, Shuolei Bu, and Zhi-Yong Wang
5. Quantitative Analysis of Plasma Proteome Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE)
Wenqiang Tang
6. Identification and Verification of Redox-Sensitive Proteins in Arabidopsis thaliana
Hai Wang, Shengbing Wang, and Yiji Xia
7. Small-Molecule Dissection of Brassinosteroid (BR) Signaling
Mirela-Corina Codreanu, Dominique Audenaert, Long Nguyen, Tom Beeckman, and Eugenia Russinova
8. A Chemical Genetics Method to Uncover Small Molecules for Dissecting the Mechanism of ABA Responses in Arabidopsis Seed Germination
Yang Zhao
9. Activation Tagging
Xiaoping Gou and Jia Li
10. Rho GTPase Activity Analysis in Plant Cells
Tongda Xu
11. Analysis of In Vivo ROP GTPase Activity at the Subcellular Level by Fluorescence Resonance Energy Transfer Microscopy
Lei Zhu and Ying Fu
12. In vivo Ubiquitination Assay by Agroinfiltration
Lijing Liu, Qingzhen Zhao, and Qi Xie
13. In Vitro Protein Ubiquitination Assay
Qingzhen Zhao, Lijing Liu, and Qi Xie
14. Genome-Wide Identification of Transcription Factor Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip) or Sequencing (ChIP-seq)
Jia-Ying Zhu, Yu Sun, and Zhi-Yong Wang
15. Smart Pooling of mRNA Samples for Efficient Transcript Profiling
Raghunandan M. Kainkaryam, Angela Bruex, Peter J. Woolf, and John Schiefelbein
16. Transient Expression Assays for Quantifying Signaling Output
Yajie Niu and Jen Sheen
17. Genome-Wide Profiling of Uncapped mRNA
Yuling Jiao and José Luis Riechmann
18. Computational Tools for Quantitative Analysis of Cell Growth Patterns and Morphogenesis in Actively Developing Plant Stem Cell Niches
Anirban Chakraborty, Ram Kishor Yadav, Min Liu, Moses Tataw, Katya Mkrtchyan, Amit Roy Chowdhury, and G. Venugopala Reddy
