1. Green-fluorescent Seed, to Evaluate Vacuolar Trafficking in Arabidopsis Seeds
Tomoo Shimada, Kentaro Fuji, Takuji Ichino; Ooi-Kock The, Yasuko KoumotoIkuko, and Hara-Nishimura
2. Detection of CRISPR/Cas9 Induced Genomic Fragment Deletions in Barley and Generation of Homozygous Edited Lines via Embryogenic Pollen Culture
Eszter Kapusi and Eva Stöger
3. Vacuolar Sorting Determinants - Isolation and Study Bruno Peixoto, Susana Pereira, Cláudia Pereira, and José Pissarra
4. Vacuole-targeted Proteins: Ins and Outs of Subcellular Localization Studies
Inês Carqueijeiro, Liuda J. Sépulveda, Angela Mosquera, Richard Payne, Cyrielle Corbin, Nicolas Papon, Thomas Dugé de Bernonville, Sébastien Besseau, Arnaud Lanoue, Gaëlle Glévarec, Marc Clastre, Benoit St-Pierre; Lucia Atehortùa, Nathalie Giglioli-Guivarc'h, Sarah E. O'Connor, Audrey Oudin, and Vincent Courdavault
5. Plant Cell Vacuoles: Staining and Fluorescent Probes
Giovanni Stefano, Luciana Renna, and Federica Brandizzi
6. Vacuolar Targeting and Characterization of Recombinant Antibodies Carolina Gabriela Ocampo and Silvana Petruccelli
7. Isolation of Vacuoles from the Leaves of the Medicinal Plant Catharanthus roseus
Inês Carqueijeiro, Henrique Noronha, Sara Bettencourt, Joana G. Guedes, Patrícia Duarte, Hernâni Gerós, and Mariana Sottomayor
8. Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells
Jorge Rodrigues, Cátia Pereira, Natacha Fontes, Hernâni Gerós, and Manuela Côrte-Real
9. Live cell Imaging and Confocal Microscopy
Luciana Renna, Giovanni Stefano, and Federica Brandizzi
10. Imaging Vacuolar Anthocyanins with Fluorescence Lifetime Microscopy (FLIM)
Alexandra Chanoca, Brian Burkel, Erich Grotewold, Kevin W. Eliceiri, and Marisa S. Otegui
11. The Use of Drugs in the Study of Vacuole Morphology and Trafficking to the Vacuole in Arabidopsis thaliana
Ricardo Tejos, Claudio Osorio-Navarro, and Lorena Norambuena
12. Use of Brefeldin A and Wortmannin to Dissect Post-Golgi Organelles Related to Vacuolar Transport in Arabidopsis thaliana Junpei Takagi and Tomohiro Uemura
13. Use Endosidin2 to Study Plant Exocytosis and Vacuolar Trafficking
Lei Huang and Chunhua Zhang
14. Visualization of RMRs (Receptor Membrane RING-H2) Dimerization in Nicotiana Benthamiana Leaves using a Bimolecular Fluorescence Complementation (BiFC) Assay Alessandro Occhialini
15. Vacuole Dynamics in Rice Cells Invaded by the Blast Fungus Magnaporthe Oryzae Kiersun Jones and Chang Hyun Khang
16. Characterization Of Plant Glycoproteins: Analysis of Plant Glycopeptide Mass Spectrometry Data with plantGlycoMS, a Package in the R Statistical Computing Environment
Margaret R. Baker, Travers Ching, David L. Tabb, and Qing X. Li
