Part I: Additional Protocols for the Study of Platelet Function
1. Intravital Imaging of Thrombus Formation in Small and Large Mouse Arteries: Experimentally-Induced Vascular Damage and Plaque Rupture In Vivo
Marijke J.E. Kuijpers and Johan W.M. Heemskerk
2. Assessment of Platelet Aggregation Responses In Vivo in the Mouse
Christopher Moore and Michael Emerson
3. Focal Cerebral Ischemia
Stefan Braeuninger, Christoph Kleinschnitz, Bernhard Nieswandt, and Guido Stoll
4. Extracorporeal Assays of Thrombosis
Lina Badimon, Teresa Padro, and Gemma Vilahur
5. Platelet Life Span and Apoptosis
Emma C. Josefsson, Michael J. White, Mark R. Dowling, and Benjamin T. Kile
6. A Live Cell Micro-Imaging Technique to Examine Platelet Calcium Signalling Dynamics Under Blood Flow
Warwick S. Nesbitt, Ian S. Harper, Simone M. Schoenwaelder, Yuping Yuan, and Shaun P. Jackson
7. Platelet Shape Change and Spreading
Joseph E. Aslan, Asako Itakura, Jacqueline M. Gertz, and Owen J.T. McCarty
8. Clot Retraction
Katherine L. Tucker, Tanya Sage, and Jonathan M. Gibbins
9. Visualization and Manipulation of the Platelet and Megakaryocyte Cytoskeleton
Jonathan N. Thon and Joseph E. Italiano
10. Measurement of Platelet Microparticles
Jeffrey I. Zwicker, Romaric Lacroix, Françoise Dignat-George, Barbara C. Furie, and Bruce Furie
11. Assessing Protein Synthesis by Platelets
Hansjörg Schwertz, Jesse W. Rowley, Neal D. Tolley, Robert A. Campbell, and Andrew S. Weyrich
12. A Rapid and Efficient Platelet Purification Protocol for Platelet Gene Expression Studies
Stefan Amisten
Part II: Additional Protocols for the Study of Megakaryocyte Function
13. Characterization of Megakaryocyte Development in the Native Bone Marrow Environment
Anita Eckly, Catherine Strassel, Jean-Pierre Cazenave, François Lanza, Catherine Léon, and Christian Gachet
14. Culture of Murine Megakaryocytes and Platelets from Fetal Liver and Bone Marrow
Harald Schulze
15. In Vitro Generation of Megakaryocytes and Platelets from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
Naoya Takayama and Koji Eto
16. Megakaryocyte and Platelet Production from Human Cord Blood Stem Cells
Amélie Robert, Valérie Cortin, Alain Garnier, and Nicolas Pineault
17. Culture of Megakaryocytes and Platelets from Subcutaneous Adipose Tissue and a Preadipocyte Cell Line
Yumiko Matsubara, Mitsuru Murata, and Yasuo Ikeda
18. Purification of Native Bone Marrow Megakaryocytes for Studies of Gene Expression
Gwen Tolhurst, Richard N. Carter, Nigel Miller, and Martyn P. Mahaut-Smith
19. Assessment of Megakaryocyte Migration and Chemotaxis
Alexandra Mazharian
Part III: Perspectives on the Study of Platelet and Megakaryocyte Function
20. Megakaryopoiesis and Thrombopoiesis: An Update on Cytokines and Lineage Surface Markers
Ming Yu and Alan B. Cantor
21. Using Zebrafish (Danio rerio) to Assess Gene Function in Thrombus Formation
Christopher M. Williams and Alastair W.
