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Poly(adp-Ribose) Polymerase

Poly(adp-Ribose) Polymerase


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About the Book

Part I: Detection and Quantification of pADPr

1. Quantitation of Poly(ADP-ribose) by Isotope Dilution Mass Spectrometry

Tabea Zubel, Rita Martello, Alexander Bürkle, and Aswin Mangerich

2. Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemistry in Human Biopsies

Eszter M. Horvath, Zsuzsanna K. Zsengellér, and Csaba Szabo

3. Detecting and Quantifying pADPr In Vivo

Yi-Chen Lai, Rajesh K. Aneja, Margaret A. Satchell, and Robert S.B. Clark

4. Compartment-Specific Poly-ADP-Ribose Formation as a Biosensor for Subcellular NAD Pools

Magali R. VanLinden, Marc Niere, Andrey A. Nikiforov, Mathias Ziegler, and Christian Dölle

5. Cell Cycle Resolved Measurements of Poly(ADP-ribose) Formation and DNA Damage Signaling by Quantitative Image-Based Cytometry

Jone Michelena and Matthias Altmeyer

Part II: Identification of Protein Targets

6. Detecting Protein ADP-Ribosylation Using a Clickable Aminooxy Probe

Rory K. Morgan and Michael S. Cohen

7. ADP-Ribosylated Peptide Enrichment and Site Identification: The Phosphodiesterase-Based Method

Casey M. Daniels, Shao-En Ong, and Anthony K.L. Leung

8. Using Clickable NAD+ Analogues to Label Substrate Proteins of PARPs

Lu Zhang and Hening Lin

9. Identification of Protein Substrates of Specific PARP Enzymes Using Analog-Sensitive PARP Mutants and a 'Clickable' NAD+ Analog

Bryan A. Gibson and W. Lee Kraus

10. Identification of ADP-Ribose Acceptor Sites on In Vitro Modified Proteins by Liquid Chromatography - Tandem Mass Spectrometry

Mario Leutert, Vera Bilan, Peter Gehrig, and Michael O. Hottiger

11. Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography -Tandem Mass Spectrometry

Sara C. Larsen, Mario Leutert, Vera Bilan, Rita Martello, Stephanie Jungmichel, Clifford Young, Michael O. Hottiger, and Michael L. Nielsen

Part III: Functional Analysis

12. Poly(ADP-Ribose)-Dependent Chromatin Remodeling in DNA Repair

Théo Lebeaupin, Rebecca Smith, Sébastien Huet, and Gyula Timinszky

13. Methods to Assess the Role of Poly(ADP-Ribose) Polymerases in Regulating Mitochondrial Oxidative Function

Edit Mikó, Tünde Kovács, Tamás Fodor, and Péter Bai

14. Approaches for Investigating Translational Regulation Controlled by PARP1: Biotin-Based UV-Crosslinking and Luciferase Reporter Assay

Yingbiao Ji

15. Methodology to Identify Poly-ADP-Ribose Polymerase 1 (PARP1)

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Product Details
  • ISBN-13: 9781493969920
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Edition: 0002-2017
  • Language: English
  • Returnable: Y
  • Spine Width: 30 mm
  • Weight: 1210 gr
  • ISBN-10: 1493969927
  • Publisher Date: 11 Jul 2017
  • Binding: Hardback
  • Height: 254 mm
  • No of Pages: 528
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm

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