Part I: Detection and Quantification of pADPr
1. Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High Performance Liquid Chromatography, and Capillary Electrophoresis
Mohsen Badiee, Audrey Boutonnet, Dat Phan, and Anthony K.L. Leung
2. Detecting and Quantifying pADPr In Vivo
Andrew M. Lamade, Yaming Chen, Carla J. Johnson, Hülya Bayır, and Robert S.B. Clark
3. Quantitative Analysis of Nuclear Poly(ADP-Ribose) Dynamics in Response to Laser-Induced DNA Damage
Christopher A. Koczor, Kate M. Saville, Rasha Q. Al-Rahahleh, Joel F. Andrews, Jianfeng Li, and Robert W. Sobol
4. Analyzing PARP1 Activity: Small Molecule Reactants and Attached Chains of Poly(ADP-Ribose)
Johannes Rudolph and Karolin Luger
5. Detecting Poly(ADP-Ribose) In Vitro and in Cells Using PAR-Trackers
Sridevi Challa, Amy L. Whitaker, and W. Lee Kraus
6. An Enzyme-Linked Immunosorbent Assay to Quantify Poly(ADP-Ribose) Level In Vivo
Chieri Ida, Sachiko Yamashita, Takayuki Eguchi, Yasuhito Kuroda, Setsu Nakae, Yoshisuke Nishi, Kazuo Kamemura, Tsuyoshi Shirai, Tamio Mizukami, Masakazu Tanaka, Joel Moss, and Masanao Miwa
7. Subcellular Quantitation of ADP-Ribosylation by High Content Microscopy
Lukas Muskalla, Anka Güldenpfennig, and Michael O. Hottiger
8. A Simple Method to Study ADP-Ribosylation Reversal: From Function to Drug Discovery
Johannes Gregor Matthias Rack and Ivan Ahel
Part II: Identification of Protein Targets
9. Immunoprecipitation Using Mono-ADP-Ribosylation Specific Antibodies
Helen Dauben and Ivan Matic
10. A Clickable NAD+ Analog-Based Assay of Poly(ADP-Ribosyl)ated Proteins
Dongsheng Yao, Heba Ahmed, and Junqi Song
11. Functional Analysis of Histone ADP-Ribosylation In Vitro and in Cells
Dan Huang, Andrea D. Edwards, Xuan Gong, and W. Lee Kraus
Part III: Functional Analysis
12. Studying the Immunomodulatory Functions of PARP1 and PARP2 in Mouse Models of Cancer
Nura Lutfi, Carlos Martínez, and José Yélamos
13. Methods for Investigating Transient Receptor Potential Melastatin-2 (TRPM2), a Cation Channel Activated by ADP-Ribose and Involved in Cell Death
Hannah K. Hall and David W. Koh
14. Methods to Assess the Role of PARPs in Regulating Mitochondrial Oxidative Function
Tünde Kovács, Boglárka Rauch, Edit Mikó, and Péter Bai
15. Characterizing ADP-Ribosylation Sites Using Af1521 Enrichment Coupled to ETD-Based Mass Spectrome
