1. Journey of Human Gametes In Vitro: 1978 to 2010
Zeev Shoham
I. Setting Up and Running an IVF Laboratory
2. Building the Laboratory
Dean E. Morbeck and Marlena Duke
3. Air Quality Management
Johan Guns, Ronny Janssens, and Martine Vercammen
4. Organizational Aspects of the Laboratory in a Tertiary Care ART Center
Nancy L. Bossert and Christopher De Jonge
5. Quality Control Management
William R. Boone and H. Lee Higdon III
6. Daily, Weekly, and Regular Preparations for the IVF Laboratory
Michael A. Britt and Klaus E. Wiemer
7. Risk and Safety in the IVF Clinic
Peter Sjoblom and Julius Hreinsson
II. IVF Laboratory Equipment and Culture Systems
8. Essential Instruments and Disposable Supplies for an IVF Laboratory
Leslie Weikert, Christa Fralick, and Klaus E. Wiemer
9. Co2 and Low-O2 Incubators
Marius Meintjes
10. IVF Workstations
Nicolas Prados and Alex C. Varghese
11. Culture Media in IVF: Decisions for the Laboratory
Jason E. Swain and Thomas B. Pool
III. In Vitro Fertilization
12. Oocyte Denuding
Roberta Maggiulli, Filippo Ubaldi, and Laura Rienzi
13. Assessment of Oocyte Quality
Basak Balaban, Turgay Barut, and Bülent Urman
14. Polarization Microscopy
Markus Montag, Maria Köster, and Hans van der Ven
15. Cumulus Cell Gene Expression in Assessment of Oocyte Quality
Dagan Wells
IV. Embryo Culture Methods
16. Short Culture: Day 1/Day 2/Day 3 Embryo Culture
Patrick Quinn
17. Extended Culture in IVF
David K. Gardner and Michelle Lane
18. In Vitro Maturation of Human Oocytes
Baris Ata, Jack Huang, and Ri-Cheng Chian
19. In Vivo Embryo Culture Device
Claude Ranoux
20. Microfluidics for Gamete Manipulation and Embryo Culture
Gary D. Smith, Charles Bormann, and Shuichi Takayama
V. Sperm Processing and Selection
21. Sperm Assessment: Traditional Approaches and Their Indicative Value
Margot Flint, Fanuel Lampiao, Ashok Agarwal, and Stefan S du Plessis
22. Sperm Assessment: Novel Approaches and Their Indicative Value
De Y. Liu, Harold Bourne, C. Garrett, G. N. Clarke, Shlomi Barak, and H. W. G. Baker
23. Sperm Processing for IVF
Ralf Henkel
24. PESA/TESA/TESE Sperm Processing
Sandro C. Esteves and Sidney Verza, Jr.
25. Processing Sperm Samples in HIV-Positive Patients
Thamara Viloria Samochin, Marcos Meseguer, Antonio Pellicer, José Remohí, and Nicolás Garrido
26. Intracytoplasmic Morphologically Selected Sperm Injection
P. Vanderzwalmen, Magnus Bach, Batsuren Baramsai, A. Neyer, Delf Schwerda, Astrid Stecher, Barbara Wirleitner, Martin Zintz, Bernard Lejeune, S. Vanderzwalmen, Nino Guy Cassuto, Mathias Zech, and Nicolas H. Zech
27. Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in the Andrology and IVF Laboratories
Gabor Huszar
28. Electrophoretic Sperm Separation
Steven Fleming and John Aitken
29. Magnetic Activated Cell Sorting of Human Spermatozoa
Enver Kerem Dirican
30. Poloscope-Based Sperm Selection
Luca Gianaroli, M. Cristina Magli, Andor Crippa, Giorgio Cavallini, Eleonora Borghi, and Anna P. Ferraretti
VI. Insemination Procedures
31. Intrauterine Insemination
Gautam N. Allahbadia and Rubina Merchant
32. Conventional IVF Insemination
Liesl Nel-Themaat, Thomas Elliott, Ching-Chien Chang, Graham Wright, and Zsolt Peter Nagy
33. Intracytoplasmic Sperm Injection
Gianpiero D. Palermo, Queenie V. Neri, Devin Monahan, Takumi Takeuchi, Peter N. Schlegel, and Zev Rosenwaks
34. Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed Fertilization
Dmitri Dozortsev and Mohammad Hossein Nasr-Esfahani
VII. Micromanipulators and Micromanipulation
35. Hydraulic Manipulators for ICSI
Hubert Joris
36. Research In
