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A Practical Manual For Plant Biotechnology

A Practical Manual For Plant Biotechnology


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About the Book

The PRACTICAL MANUAL FOR PLANT BIOTECHNOLOGY, has been written In view of the increasing number of students In this discipline. There are a fair number of text books avail able. which cover different aspects of biotechnology, but there Is a dearth of practical guides for the students. It Is the first attempt of authors to put together certain technical fr and experimental aspects of plant biotechnology which will help post-graduate students to carry out such studies In their laboratories. However. coverage of all possible experiments, techniques and details of Instruments is at present beyond the purview of the book. The first four chapters of the manual contain the basics about the Instruments used In these studies and the methodology followed for sterilization and preparation of media. Chapters 5 to 17 are devoted to some laboratory exercises in Biotechnology. Glossary of Important terms and a cursory list of Indian laboratories Involved In biotechnological studies Is followed by the list of books referred In this manual and suggested for further reading. We welcome critical comments from the readers.Foreword Preface Acknowledgement Introduction Methods of Sterilization Instruments Experimental techniques 1. Media Preparation 2. Sterilization of Plant Material 3. Callus Induction & Regeneration 4. Methods of Growth Measurements In Callus Cultures 5. Isolation of Agriculturally Useful Mutants 6. Production of Pathogen - Free Plants 7. Isolation of Secondary Metabolites from tissue Cultures (a) Alkaloids (b) Phenolics 8. Mechanical Isolation of Protoplast 9. Enzymatic Isolation of Protoplasts Culture 10. Protoplast Fusion 11. Anther Culture & Haploid Production. 12. Protein Separation by Electrophoresis 13. Isolation of Deoxy-Ribonucleic Acid (DNA) from (a) Bacteria (Genomic) (b) Bacteria (Plasmid) (C) Virus (d) Plants 4. Digestion of DNA with Restriction Enzymes 15. Agrobacterium mediated Transformations Biotechnology Centres In India References & further .


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Product Details
  • ISBN-13: 9788123904511
  • Publisher: CBS Publishers & Distributors
  • Publisher Imprint: Cbs Publishers & Distributors
  • Edition: 1
  • Language: English
  • Spine Width: 4.06 mm
  • Width: 135.89 mm
  • ISBN-10: 8123904517
  • Publisher Date: 1996
  • Binding: Paperback
  • Height: 212.09 mm
  • No of Pages: 79
  • Weight: 117.93 gr


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