Produção de HFS pela glucose isomerase isolada de E.coli DH5 Alpha
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Produção de HFS pela glucose isomerase isolada de E.coli DH5 Alpha

Produção de HFS pela glucose isomerase isolada de E.coli DH5 Alpha


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About the Book

A xilose/glucose isomerase é uma enzima industrial importante, que tem sido utilizada para a conversão de glucose em frutose há décadas. Esta investigação centra-se na produção de glucose isomerase a partir de Escherichia coli DH5α. Os estudos de otimização da fermentação mostraram que uma maior quantidade de extrato de levedura e de triptona aumentava o crescimento celular e que a temperatura óptima para o crescimento era de 37°C. A purificação parcial da xilose isomerase foi efectuada utilizando tratamento térmico e precipitação com sulfato de amónio. 80% do valor de corte do sal produziu uma atividade máxima para o concentrado enzimático. A xilose isomerase apresentou actividades máximas à temperatura de 75 °C e pH 8. A atividade enzimática em condições óptimas foi de 5,0 U/min/ml para E. coli DH5α. A adição de iões de cobalto e xilose aumentou a atividade de 10 a 20 vezes. A atividade específica para E. coli DH5α foi de 60,5 nmol/min/mg de proteína. Apenas 8% do xarope de D-frutose máximo foi produzido utilizando 100 mM de D-glucose como substrato após 14 dias de incubação. Deste modo, a E. coli DH5α não é a melhor candidata para a produção de xarope de frutose elevado.


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Product Details
  • ISBN-13: 9786206857945
  • Publisher: KS Omniscriptum Publishing
  • Binding: Paperback
  • Language: Portuguese
  • Returnable: N
  • Weight: 145 gr
  • ISBN-10: 6206857948
  • Publisher Date: 21 Nov 2023
  • Height: 229 mm
  • No of Pages: 56
  • Spine Width: 3 mm
  • Width: 152 mm


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