1. Expression and Purification of Recombinant Proteins in Escherichia Coli with a His6 or Dual His6-MBP Tag
Sreejith Raran-Kurussi and David S. Waugh
2. Protein Crystallization
Alexander McPherson
3. Advanced Methods of Protein Crystallization
Abel Moreno
4. The 'Sticky Patch' Model of Crystallization and Modification of Proteins for Enhanced Crystallizability
Zygmunt S. Derewenda and Adam Godzik
5. Crystallization of Membrane Proteins: and Overview
Andrii Ishchenko, Enrique Abola, and Vadim Cherezov
6. Locating and Visualizing Crystals for X-ray Diffraction Experiments
Michael Becker, David J. Kissick, and Craig M. Ogata
7. Collection of X-ray Diffraction Data from Macromolecular Crystals
Zbigniew Dauter
8. Identifying and Overcoming Crystal Pathologies: Disorder and Twinning
Michael C. Thompson
9. Applications of X-ray Micro-beam for Data Collection
Ruslan Sanishvili and Robert F. Fischetti
10. Serial Synchrotron X-ray Crystallography (SSX)
Kay Diederichs and Meitian Wang
11. Time-Resolved Macromolecular Crystallography at Modern X-ray Sources
Marius Schmidt
12. Structure Determination Using X-ray Free-electron Laser Pulses
Henry N. Chapman
13. Processing of XFEL Data
Thomas A. White
14. Many Ways to Derivatize Macromolecules and their Crystals for Phasing
Miroslawa Dauter and Zbigniew Dauter
15. Experimental Phasing: Substructure Solution and Density Modification as Implemented in SHELX
Andrea Thorn
16. Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis
Qun Liu and Wayne A. Hendrickson
17. Long-Wavelength X-ray Diffraction and its Applications in Macromolecular Crystallography
Manfred S. Weiss
18. Acknowledging Errors: Advanced Molecular Replacement with Phaser
Airlie J. McCoy
19. Rosetta Structure Prediction as a Tool for Solving Difficult Molecular Replacement Problems
Frank DiMaio
20. Radiation Damage in Macromolecular Crystallography
Elspeth F. Garman and Martin Weik
21. Boxes of Model Building and Visualization Dusan Turk
22. Structure Refinement at Atomic Resolution
Mariusz Jaskolski
23. Low Resolution Refinement of Atomic Models against Crystallographic Data
Robert A. Nicholls, Oleg Kovalevskiy, and Garib N. Murshudov
24. Stereochemistry and Validation of Macromolecular Structures
Alexander Wlodawer
25. Validation of Protein-Ligand Crystal Structure Models: Small Molecule and Peptide Ligands
Edwin Pozharski, Marc C. Deller and Bernhard Rupp
26. Protein Data Bank - the Single Global Macromolecular Structure Archive Managed by the Worldwide Protein Data Bank
Stephen K. Burley Helen M. Berman, Gerard J. Kleywegt, John L. Markley, Haruki Nakamura, and Sameer Velankar
27. Databases, Repositories and Other Data Resources in Structural Biology
Heping Zheng, Przemyslaw J. Porebski, Marek Grabowski, David R. Cooper, and Wladek Minor
