Part I: Protein-Detecting Analytical Microarrays
1. Detecting and Quantifying Multiple Proteins in Clinical Samples in High-Throughput Using Antibody Microarrays
Tanya Knickerbocker and Gavin MacBeath
2. Analysis of Serum Protein Glycosylation with Antibody-Lectin Microarray for High-Throughput Biomarker Screening
Chen Li and David M. Lubman
3. Antibody Suspension Bead Arrays
Jochen M. Schwenk and Peter Nilsson
4. Reverse Protein Arrays Applied to Host-Pathogen Interaction Studies
Víctor J. Cid, Ekkehard Kauffmann, and María Molina
5. Identification and Optimization of DNA Aptamer Binding Regions Using DNA Microarrays
Nicholas O. Fischer and Theodore M. Tarasow
6. Recombinant Lectin Microarrays for Glycomic Analysis
Daniel C. Propheter, Ku-Lung Hsu, and Lara K. Mahal
Part II: Antigen Microarrays for Immunoprofiling
7. Recombinant Antigen Microarrays for Serum/Plasma Antibody Detection
Persis P. Wadia, Bita Sahaf, and David B. Miklos
8. SPOT Synthesis as a Tool to Study Protein-Protein Interactions
Dirk F.H. Winkler, Heiko Andresen, and Kai Hilpert
9. Native Antigen Fractionation Protein Microarrays for Biomarker Discovery
Robert J. Caiazzo, Jr., Dennis J. O'Rourke, Timothy J. Barder, Bryce P. Nelson, and Brian C.-S. Liu
10. Immunoprofiling Using NAPPA Protein Microarrays
Sahar Sibani and Joshua LaBaer
Part III: Protein Function Microarrays
11. High-Throughput Mammalian Two-Hybrid Screening for Protein-Protein Interactions Using Transfected Cell Arrays (CAPPIA)
Andrea Fiebitz and Dominique Vanhecke
12. Protein-Protein Interactions: An Application of Tus-Ter Mediated Protein Microarray System
Kalavathy Sitaraman and Deb K. Chatterjee
13. Kinase Substrate Interactions
Michael G. Smith, Jason Ptacek, and Michael Snyder
14. A Functional Protein Microarray Approach to Characterizing Posttranslational Modifications on Lysine Residues
Jun Seop Jeong, Hee-Sool Rho, and Heng Zhu
Part IV: Strategies for Validation of Candidate Targets
15. Multiplexed Detection of Antibodies Using Programmable Bead Arrays
Karen S. Anderson
16. A Co-Precipitation-Based Validation Methodology for Interactions Identified Using Protein Microarrays
Ovidiu Marina, Jonathan S. Duke-Cohan, and Catherine J. Wu
Part V: Generation of Proteomic Libraries
17. Development of Expression-Ready Constructs for Generation of Proteomic Libraries
Charles Yu, Kenneth H. Wan, Ann S. Hammonds, Mark Stapleton, Joseph W. Carlson, and Susan E. Celniker
Part VI: Detection Methods
18. Reverse Phase Protein Microarrays: Fluorometric and Colorimetric Detection
Rosa I. Gallagher, Alessandra Silvestri, Emanuel F. Petricoin III, Lance A. Liotta, and Virginia Espina
19. Förster Resonance Energy Transfer Methods for Quantification of Protein-Protein Interactions on Microarrays
Michael Schäferling and Stefan Nagl
20. Label-Free Detection with Surface Plasmon Resonance Imaging
Christopher Lausted, Zhiyuan Hu, and
